Human Anti-BRD2 Recombinant Antibody (clone 2506W1328)
CAT#: VS-0625-FY1433
This recombinant antibody is a human IgG1 antibody, generated using recombinant antibody (rAb) phage display. It has been thoroughly tested for its specificity and affinity, recognizing the BRD2 protein under non-denaturing conditions. While specific applications are not listed, this antibody offers a precise tool for BRD2 research. The antibody targets an epitope spanning amino acids 71-194 of the BRD2 protein, making it highly valuable for studying BRD2 in its native conformation and for developing custom assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human testis
Immunohistochemical staining of human testis shows strong nuclear positivity in spermatogonia.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42816/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42816/486_H3_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42816/490_H3_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42816/509_H3_1_red_green.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42816/92904_A_7_3.jpg
Normal Tissue
Figure 6 Testis
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42816/92904_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42816/92904_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000204256-BRD2
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Specifications
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- The antibody reacts with human BRD2.
- Species Reactivity
- Human
- Clone
- 2506W1328
- Applications
- ELISA, Immunoprecipitation Mass Spectrometry
Product Property
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (over 6 months). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Optimal dilutions for assays should be determined by the user.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "BRD2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-246 | Human Anti-BRD2 Recombinant Antibody (clone D380) | ELISA, IP-MS | Human IgG1 |
| MRO-0191-CN | Rabbit Anti-BRD2 Recombinant Antibody (clone JG40-42) | WB, IF, IHC, FC | Rabbit IgG |
| HPAB-0353-FY | Mouse Anti-BRD2 Recombinant Antibody (HPAB-0353-FY) | WB, ELISA, Cyt, FuncS | Mouse IgG1 |
| HPAB-0354-FY | Mouse Anti-BRD2 Recombinant Antibody (HPAB-0354-FY) | ELISA, Cyt | Mouse IgG2a, κ |
| VS7-HM257 | Mouse Anti-BRD2 Recombinant Antibody (clone 7C1B10) | WB, FCM | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0383 | Hi-Affi™ Rabbit Anti-BRD2 Recombinant Antibody (clone DS383AB) | WB, FC, IF, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0355-FY | Mouse Anti-BRD2 Recombinant Antibody (HPAB-0355-FY) | Neut, ELISA, Cyt, FuncS | Mouse IgM, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0353-FY-S(P) | Mouse Anti-BRD2 Recombinant Antibody; scFv Fragment (HPAB-0353-FY-S(P)) | WB, ELISA | Mouse scFv |
| HPAB-0354-FY-S(P) | Mouse Anti-BRD2 Recombinant Antibody; scFv Fragment (HPAB-0354-FY-S(P)) | ELISA | Mouse scFv |
| HPAB-0355-FY-S(P) | Mouse Anti-BRD2 Recombinant Antibody; scFv Fragment (HPAB-0355-FY-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0353-FY-F(E) | Mouse Anti-BRD2 Recombinant Antibody; Fab Fragment (HPAB-0353-FY-F(E)) | WB, ELISA | Mouse Fab |
| HPAB-0354-FY-F(E) | Mouse Anti-BRD2 Recombinant Antibody; Fab Fragment (HPAB-0354-FY-F(E)) | ELISA | Mouse Fab, κ |
| HPAB-0355-FY-F(E) | Mouse Anti-BRD2 Recombinant Antibody; Fab Fragment (HPAB-0355-FY-F(E)) | ELISA | Mouse Fab, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC107 | CytoStream™ Rabbit Anti-BRD2 Recombinant Antibody (VS13-YC107) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
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