Mouse Anti-BRD2 Recombinant Antibody (clone 7C1B10)
CAT#: VS7-HM257
Mouse Anti-BRD2 Recombinant Antibody (clone 7C1B10) is an mouse IgG2b antibody that recognizes the Human, Rat BRD2 by WB, FCM.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human testis
Immunohistochemical staining of human testis shows strong nuclear positivity in spermatogonia.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42816/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42816/486_H3_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42816/490_H3_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42816/509_H3_1_red_green.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42816/92904_A_7_3.jpg
Normal Tissue
Figure 6 Testis
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42816/92904_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42816/92904_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000204256-BRD2
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Specifications
- Immunogen
- Purified recombinant human BRD2 (AA: 227-364) fragment expressed in E. Coli.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human, Rat BRD2
- Species Reactivity
- Human, Rat
- Clone
- 7C1B10
- Applications
- WB, FCM
- Conjugate
- None
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity-chromatography
- Format
- Liquid
- Molecular Weight
- 88kDa
- Concentration
- Please refer to the tube label.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide.
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice pack
Target
- Alternative Names
- BRD2; FSH; NAT; RNF3; FSRG1; RING3; D6S113E; bromodomain-containing protein 2; O27.1.1; bromodomain-containing 2; female sterile homeotic-related gene 1; really interesting new gene 3 protein
- Gene ID
- 6046
- UniProt ID
- A0A024RCR5
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| HPAB-0353-FY | Mouse Anti-BRD2 Recombinant Antibody (HPAB-0353-FY) | WB, ELISA, Cyt, FuncS | Mouse IgG1 |
| VS3-FY1670 | Rabbit Anti-BRD2 Recombinant Antibody (clone R05-7E0) | WB | Rabbit IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0383 | Hi-Affi™ Rabbit Anti-BRD2 Recombinant Antibody (clone DS383AB) | WB, FC, IF, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0355-FY | Mouse Anti-BRD2 Recombinant Antibody (HPAB-0355-FY) | Neut, ELISA, Cyt, FuncS | Mouse IgM, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0353-FY-S(P) | Mouse Anti-BRD2 Recombinant Antibody; scFv Fragment (HPAB-0353-FY-S(P)) | WB, ELISA | Mouse scFv |
| HPAB-0354-FY-S(P) | Mouse Anti-BRD2 Recombinant Antibody; scFv Fragment (HPAB-0354-FY-S(P)) | ELISA | Mouse scFv |
| HPAB-0355-FY-S(P) | Mouse Anti-BRD2 Recombinant Antibody; scFv Fragment (HPAB-0355-FY-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0353-FY-F(E) | Mouse Anti-BRD2 Recombinant Antibody; Fab Fragment (HPAB-0353-FY-F(E)) | WB, ELISA | Mouse Fab |
| HPAB-0354-FY-F(E) | Mouse Anti-BRD2 Recombinant Antibody; Fab Fragment (HPAB-0354-FY-F(E)) | ELISA | Mouse Fab, κ |
| HPAB-0355-FY-F(E) | Mouse Anti-BRD2 Recombinant Antibody; Fab Fragment (HPAB-0355-FY-F(E)) | ELISA | Mouse Fab, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC107 | CytoStream™ Rabbit Anti-BRD2 Recombinant Antibody (VS13-YC107) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
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