Mouse Anti-BRIP1 Recombinant Antibody (VS3-FY2637) (CAT#: VS3-FY2637)
The mouse anti-BRIP1 recombinant monoclonal antibody has been developed to target BRIP1 protein, a calculated weight of 140.8 kDa. It exhibits cross-reactivity with human. This monoclonal antibody is potential for conducting research on Fanconi Anemia, Complementation Group J and Breast cancer. The antibody can be utilized for detection of BRIP1 by Immunohistochemistry, Flow cytometry, ELISA.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5474/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5474/if_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5474/16186_B_8_5.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5474/16186_B_8_5.jpg
Figure 3 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5474/16186_A_8_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5474/16186_A_8_3.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5474/16186_A_8_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5474/16186_A_8_5.jpg
Figure 5 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5474/16186_A_6_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5474/16186_A_6_6.jpg
Figure 6 Lymph node
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5474/16186_A_7_8.jpg
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5474/16186_A_7_8.jpg
Figure 7 RNA cell line category: Cell line enhanced (MCF7)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000136492-BRIP1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000136492-BRIP1
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- BRCA1 interacting protein C-terminal helicase 1 of human
- Species Reactivity
- Human
- Applications
- IHC, FC, ELISA
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 140.8 kDa.
- Related Disease
- Fanconi Anemia, Complementation Group J and Breast Cancer
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry: 1/50-1/200
Flow cytometry: 1/50-1/200
ELISA: 1/1000-1/10000
Target
- Alternative Names
- BRCA1 Interacting Protein C-Terminal Helicase 1; BRCA1-Associated C-Terminal Helicase 1; BRCA1/BRCA2-Associated Helicase 1; ATP-Dependent RNA Helicase BRIP1; BACH1; FANCJ; BRCA1-Interacting Protein C-Terminal Helicase 1; BRCA1-Binding Helicase-Like Protein BACH1
- Gene ID
- 83990
- Sequence Similarities
- Belongs to the DEAD box helicase family. DEAH subfamily.
- Cellular Localization
- Nucleus
Cytoplasm
- Post Translation Modifications
- Phosphorylated.
Phosphorylation is necessary for interaction with BRCA1, and is cell-cycle regulated.
Acetylation at Lys-1249 facilitates DNA end processing required for repair and checkpoint signaling.
Ubiquitination at Lys862
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011523634.1; XP_011523635.1; XP_011523636.1; XP_011523637.1; XP_011523638.1
- Function
- Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination.
Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "BRIP1"
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IgG Antibodies
Rabbit Monoclonal Antibodies
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0884MZ | Mouse Anti-BRIP1 Recombinant Antibody (clone 4H22) | WB | Mouse IgG1 |
| VS7-HM258 | Mouse Anti-BRIP1 Recombinant Antibody (clone 1B8F9) | IHC, FCM | Mouse IgG1 |
| VS7-HM259 | Mouse Anti-BRIP1 Recombinant Antibody (clone 4C6C8) | IHC, FCM | Mouse IgG1 |
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