Mouse Anti-BRIP1 Recombinant Antibody (VS3-FY2637)
CAT#: VS3-FY2637
The mouse anti-BRIP1 recombinant monoclonal antibody has been developed to target BRIP1 protein, a calculated weight of 140.8 kDa. It exhibits cross-reactivity with human. This monoclonal antibody is potential for conducting research on Fanconi Anemia, Complementation Group J and Breast cancer. The antibody can be utilized for detection of BRIP1 by Immunohistochemistry, Flow cytometry, ELISA.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5474/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5474/16186_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5474/16186_A_8_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5474/16186_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5474/16186_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5474/16186_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (MCF7)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000136492-BRIP1
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- BRCA1 interacting protein C-terminal helicase 1 of human
- Species Reactivity
- Human
- Applications
- IHC, FC, ELISA
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 140.8 kDa.
- Related Disease
- Fanconi Anemia, Complementation Group J and Breast Cancer
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry: 1/50-1/200
Flow cytometry: 1/50-1/200
ELISA: 1/1000-1/10000
Target
- Alternative Names
- BRCA1 Interacting Protein C-Terminal Helicase 1; BRCA1-Associated C-Terminal Helicase 1; BRCA1/BRCA2-Associated Helicase 1; ATP-Dependent RNA Helicase BRIP1; BACH1; FANCJ; BRCA1-Interacting Protein C-Terminal Helicase 1; BRCA1-Binding Helicase-Like Protein BACH1
- Gene ID
- 83990
- Sequence Similarities
- Belongs to the DEAD box helicase family. DEAH subfamily.
- Cellular Localization
- Nucleus
Cytoplasm
- Post Translation Modifications
- Phosphorylated.
Phosphorylation is necessary for interaction with BRCA1, and is cell-cycle regulated.
Acetylation at Lys-1249 facilitates DNA end processing required for repair and checkpoint signaling.
Ubiquitination at Lys862
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011523634.1; XP_011523635.1; XP_011523636.1; XP_011523637.1; XP_011523638.1
- Function
- Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination.
Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "BRIP1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0884MZ | Mouse Anti-BRIP1 Recombinant Antibody (clone 4H22) | WB | Mouse IgG1 |
| VS7-HM258 | Mouse Anti-BRIP1 Recombinant Antibody (clone 1B8F9) | IHC, FCM | Mouse IgG1 |
| VS7-HM259 | Mouse Anti-BRIP1 Recombinant Antibody (clone 4C6C8) | IHC, FCM | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0386 | Hi-Affi™ Rabbit Anti-BRIP1 Recombinant Antibody (clone DS386AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY833 | Anti-BRIP1 Immunohistochemistry Kit | IHC |
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