Rabbit Anti-BUB1B Recombinant Antibody (clone 7H4) (CAT#: ZG-0575J)
This product is a rabbit antibody that recognizes BUB1B.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-BUB1B Antibody (ZG-0575J) in WB
Western Blot
Positive WB detected in: K562 whole cell lysate, U-251 whole cell lysate
All lanes: BubR1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 120, 106, 122 kDa
Observed band size: 125 kDa
FC
Figure 2 Rabbit Anti-BUB1B Antibody (ZG-0575J) in FC
Overlay histogram showing Hela cells stained with ZG-0575J (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.
Specifications
- Immunogen
- A synthesized peptide derived from human BubR1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human BUB1B
- Species Reactivity
- Human
- Clone
- 7H4
- Applications
- ELISA, WB, FC
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry.
WB:1:500-1:5000, FC:1:20-1:200
Target
- Alternative Names
- MVA1; SSK1; BUBR1; Bub1A; MAD3L; hBUBR1; BUB1beta; mitotic checkpoint serine/threonine-protein kinase BUB1 beta; BUB1B, mitotic checkpoint serine/threonine kinase; MAD3/BUB1-related protein kinase; budding uninhibited by benzimidazoles 1 homolog beta; mitotic checkpoint kinase MAD3L
- Gene ID
- 701
- UniProt ID
- O60566
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2965z | Mouse Anti-BUB1B Recombinant Antibody (clone 11G9) | WB, ICC, IF, IP | Mouse IgG1 |
| MRO-1702-CN | Rabbit Anti-BUB1B Polyclonal Antibody (MRO-1702-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0946F | Sheep Anti-Bub1b Antibody (ZG-0946F) | WB | Sheep IgG |
| VS-0422-LC134 | Mouse Anti-BUB1B Recombinant Antibody (clone mN129A/6R) | ICC, WB | Mouse IgG2a |
| VS-0422-LC135 | Human Anti-BUB1B Recombinant Antibody (clone hN129A/6R) | ICC, WB | Human IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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