Rabbit Anti-BUB1B Recombinant Antibody (clone 7H4)
CAT#: ZG-0575J
This product is a rabbit antibody that recognizes BUB1B.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-BUB1B Antibody (ZG-0575J) in WB
Western Blot
Positive WB detected in: K562 whole cell lysate, U-251 whole cell lysate
All lanes: BubR1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 120, 106, 122 kDa
Observed band size: 125 kDa
FC
Figure 2 Rabbit Anti-BUB1B Antibody (ZG-0575J) in FC
Overlay histogram showing Hela cells stained with ZG-0575J (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8419/68_H6_1_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000156970-BUB1B
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Specifications
- Immunogen
- A synthesized peptide derived from human BubR1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human BUB1B
- Species Reactivity
- Human
- Clone
- 7H4
- Applications
- ELISA, WB, FC
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry.
WB:1:500-1:5000, FC:1:20-1:200
Target
- Alternative Names
- MVA1; SSK1; BUBR1; Bub1A; MAD3L; hBUBR1; BUB1beta; mitotic checkpoint serine/threonine-protein kinase BUB1 beta; BUB1B, mitotic checkpoint serine/threonine kinase; MAD3/BUB1-related protein kinase; budding uninhibited by benzimidazoles 1 homolog beta; mitotic checkpoint kinase MAD3L
- Gene ID
- 701
- UniProt ID
- O60566
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "BUB1B"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2965z | Mouse Anti-BUB1B Recombinant Antibody (clone 11G9) | WB, ICC, IF, IP | Mouse IgG1 |
| ZG-0946F | Sheep Anti-Bub1b Antibody (ZG-0946F) | WB | Sheep IgG |
| VS-0422-LC134 | Mouse Anti-BUB1B Recombinant Antibody (clone mN129A/6R) | ICC, WB | Mouse IgG2a |
| VS-0422-LC135 | Human Anti-BUB1B Recombinant Antibody (clone hN129A/6R) | ICC, WB | Human IgG1 |
| VS-0422-LC136 | Rabbit Anti-BUB1B Recombinant Antibody (clone rN129A/6R) | ICC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0396 | Hi-Affi™ Rabbit Anti-BUB1B Recombinant Antibody (clone DS396AB) | WB, IHC-P, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY269 | Anti-BUB1B Immunohistochemistry Kit | IHC | |
| VS-0525-XY862 | Anti-Mouse BUB1B Immunohistochemistry Kit | IHC | |
| VS-0525-XY861 | Anti-Human BUB1B Immunohistochemistry Kit | IHC |
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