Rabbit Anti-BUB1B Polyclonal Antibody (MRO-1702-CN)
CAT#: MRO-1702-CN
This antibody is a rabbit polyclonal antibody that is capable of binding to BUB1B.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8419/68_H6_1_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000156970-BUB1B
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Specifications
- Immunogen
- Recombinant protein within human BubR1 330-600 aa.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human BUB1B (BUB1 Mitotic Checkpoint Serine/Threonine Kinase B)
- Species Reactivity
- Human
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunofluorescence; Immunohistochemistry; Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein affinity purified
- Format
- Liquid
- Buffer
- 1*TBS, pH 7.4, 0.5% BSA, 50% Glycerol
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- BUB1 Mitotic Checkpoint Serine/Threonine Kinase B; MAD3/BUB1-Related Protein Kinase; Mitotic Checkpoint Kinase MAD3L; HBUBR1; BUBR1; MAD3L; SSK1; Budding Uninhibited By Benzimidazoles 1 (Yeast Homolog), Beta; Budding Uninhibited By Benzimidazoles 1 Homolog Beta (Yeast);
- Gene ID
- 701
- UniProt ID
- O60566
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Good BUB1B Antibody
This rabbit anti-BUB1B antibody is a solid reagent. We are using it for Western Blot (WB) and Immunohistochemistry (IHC) to study cell cycle regulation, and it works well for both. The validation for human, mouse, and rat species is also very convenient.
Works in Mouse and Human
I appreciate that this rabbit antibody for BUB1B is validated for cross-reactivity with human, mouse, and rat. This allows us to use the same reagent in both our human cell lines and our mouse models for our cell cycle research. It provides consistent results in WB and IF.
Q&As
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What species is this antibody validated for?
A: This rabbit antibody (MRO-1702-CN) has been validated for reactivity with human. This makes it a versatile tool for researchers who work using the common research models. The antibody is a Rabbit IgG.
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What is the target BUB1B, and what applications can I use this antibody for?
A: This antibody targets BUB1B (Bub1 budding uninhibited by benzimidazoles 1 homolog beta), a key protein in cell cycle research. It is a critical component of the spindle assembly checkpoint, which ensures chromosomes are properly aligned before cell division. Our data confirms this antibody is validated for Immunofluorescence (IF), Western Blot (WB), and Immunohistochemistry (IHC).
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "BUB1B"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2965z | Mouse Anti-BUB1B Recombinant Antibody (clone 11G9) | WB, ICC, IF, IP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0945MZ | Mouse Anti-BUB1B Recombinant Antibody (clone CBLS-180) | IHC-P | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0396 | Hi-Affi™ Rabbit Anti-BUB1B Recombinant Antibody (clone DS396AB) | WB, IHC-P, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0946F | Sheep Anti-Bub1b Antibody (ZG-0946F) | WB | Sheep IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0575J | Rabbit Anti-BUB1B Recombinant Antibody (clone 7H4) | ELISA, WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0422-LC134 | Mouse Anti-BUB1B Recombinant Antibody (clone mN129A/6R) | ICC, WB | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY269 | Anti-BUB1B Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY862 | Anti-Mouse BUB1B Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY861 | Anti-Human BUB1B Immunohistochemistry Kit | IHC |
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