Human Anti-C. botulinum BoNT/A Recombinant Antibody (clone 4LCA) (CAT#: HPAB-1967-FY)
This product is a recombinant antibody that recognizes BoNT serotype A. The monoclonal antibody 4LCA reacts with the epitope of BoNT serotype A. Inhibition of the catalytic activity of the BoNT/A metalloprotease by the 4LCA antibody in vitro.
Specific Inquiry
IF
Figure 1 The effect of BoNT/A-specific antibodies on endocytosis of BoNT/A by Neuro-2a cells.
Neuro-2a cells were cultured withfluorescently-labeled BoNT/A (red), with or without human antibody (green), and visualized by confocal microscopy.
Adekar, S. P., Takahashi, T., Jones, R. M., Al-Saleem, F. H., Ancharski, D. M., Root, M. J., ... & Dessain, S. K. (2008). Neutralization of botulinum neurotoxin by a human monoclonal antibody specific for the catalytic light chain. Plos one, 3(8).
Inhib
Figure 2 Inhibition of the catalytic activity of the BoNT/Ametalloprotease by the 4LCA antibody.
In this fluores-cence resonance energy transfer assay (FRET), cleavage of the SNAPtideby BoNT/A LC un-quenches FITC fluorescence, which is quantified asarbitrary fluorescence units. In triplicate samples, the SNAPtide wasincubated with BoNT/A alone or with the 6A or 4LCA antibodies andfluorescence was measured. Standard deviation (s.d.) is indicated byerror bars.
Adekar, S. P., Takahashi, T., Jones, R. M., Al-Saleem, F. H., Ancharski, D. M., Root, M. J., ... & Dessain, S. K. (2008). Neutralization of botulinum neurotoxin by a human monoclonal antibody specific for the catalytic light chain. Plos one, 3(8).
Specifications
- Host Species
- Human
- Type
- Human IgG1, λ
- Specificity
- C. botulinum BoNT/A
- Species Reactivity
- C. botulinum
- Clone
- 4LCA
- Applications
- Inhib, IF
- Related Disease
- Botulinum Toxin
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The BONT/A antibody has been reported in applications of Inhibition, Immunofluorescence.
Target
- Alternative Names
- Botulinum neurotoxins serotype A; BoNT serotype A
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Single Domain Antibody Products
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1537-sdAb | Recombinant Anti-C. botulinum BoNT/A VHH Single Domain Antibody | FUNC, FA, IP, ChiP | Llama VHH |
| HPAB-0684-YJ-VHH | Camelid Anti-C. botulinum BoNT/A Recombinant Single Domain Antibody (HPAB-0684-YJ-VHH) | ELISA, Neut | Camelid VHH |
| HPAB-0685-YJ-VHH | Camelid Anti-C. botulinum BoNT/A Recombinant Single Domain Antibody (HPAB-0685-YJ-VHH) | ELISA | Camelid VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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