Human Anti-C. botulinum BoNT/A Recombinant Antibody (clone 4LCA) (CAT#: HPAB-1967-FY)
This product is a recombinant antibody that recognizes BoNT serotype A. The monoclonal antibody 4LCA reacts with the epitope of BoNT serotype A. Inhibition of the catalytic activity of the BoNT/A metalloprotease by the 4LCA antibody in vitro.
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IF
Figure 1 The effect of BoNT/A-specific antibodies on endocytosis of BoNT/A by Neuro-2a cells.
Neuro-2a cells were cultured withfluorescently-labeled BoNT/A (red), with or without human antibody (green), and visualized by confocal microscopy.
Adekar, S. P., Takahashi, T., Jones, R. M., Al-Saleem, F. H., Ancharski, D. M., Root, M. J., ... & Dessain, S. K. (2008). Neutralization of botulinum neurotoxin by a human monoclonal antibody specific for the catalytic light chain. Plos one, 3(8).
Inhib
Figure 2 Inhibition of the catalytic activity of the BoNT/Ametalloprotease by the 4LCA antibody.
In this fluores-cence resonance energy transfer assay (FRET), cleavage of the SNAPtideby BoNT/A LC un-quenches FITC fluorescence, which is quantified asarbitrary fluorescence units. In triplicate samples, the SNAPtide wasincubated with BoNT/A alone or with the 6A or 4LCA antibodies andfluorescence was measured. Standard deviation (s.d.) is indicated byerror bars.
Adekar, S. P., Takahashi, T., Jones, R. M., Al-Saleem, F. H., Ancharski, D. M., Root, M. J., ... & Dessain, S. K. (2008). Neutralization of botulinum neurotoxin by a human monoclonal antibody specific for the catalytic light chain. Plos one, 3(8).
Specifications
- Host Species
- Human
- Type
- Human IgG1, λ
- Specificity
- C. botulinum BoNT/A
- Species Reactivity
- C. botulinum
- Clone
- 4LCA
- Applications
- Inhib, IF
- Related Disease
- Botulinum Toxin
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The BONT/A antibody has been reported in applications of Inhibition, Immunofluorescence.
Target
- Alternative Names
- Botulinum neurotoxins serotype A; BoNT serotype A
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1537-sdAb | Recombinant Anti-C. botulinum BoNT/A VHH Single Domain Antibody | FUNC, FA, IP, ChiP | Llama VHH |
| HPAB-0684-YJ-VHH | Camelid Anti-C. botulinum BoNT/A Recombinant Single Domain Antibody (HPAB-0684-YJ-VHH) | ELISA, Neut | Camelid VHH |
| HPAB-0685-YJ-VHH | Camelid Anti-C. botulinum BoNT/A Recombinant Single Domain Antibody (HPAB-0685-YJ-VHH) | ELISA | Camelid VHH |
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For Research Use Only. Not For Clinical Use.
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