Mouse Anti-CA12 Recombinant Antibody (clone 14D6)
CAT#: VS-0323-CJ3
This mouse anti-CA12 antibody (clone 14D6) is a product that recognizes the recombinant extracellular domain of Human carbonic anhydrase XII (CA12).
Published Data
Gene Expression
FC
Figure 1 The binding of MAb 14D6 with native CA XII localized on the surface of live A498 cells was investigated by flow cytometry.
Inhib
Figure 2 Inhibition of CO2 hydratase activity of CA XIIHEK by the MAb 14D6.
WB
Figure 3 Analysis of the lysates of E. coli expressing overlapping fragments of CA XII with the 14D6-Derived scFv-Fc and anti-human Fc secondary antibody by Western blot.
ELISA
Figure 4 Apparent dissociation constants (Kd, M) of MAb 14D6, RAb scFv, and RAb scFv-Fc determined by an indirect ELISA.
FuncS
Figure 5 MAb 14D6 showed greater inhibitory activity on A498 cell migration.
Figure 6 Effect of MAb 14D6 and isotype control (TSPyV MAb 20D3) on human lung adenocarcinoma A498 and A549 spheroids.
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Normal Tissue
Figure 1 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25181/53836_A_9_3.jpg
Normal Tissue
Figure 2 Kidney
Collecting ducts Staining: Medium Intensity: Strong Quantity: <25% Distal tubules Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25181/53836_A_8_5.jpg
Normal Tissue
Figure 3 Skin
Cells in basal layer Staining: High Intensity: Strong Quantity:>75% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Eccrine glands Staining: Medium Intensity: Moderate Quantity: 75%-25% Fibrohistiocytic cells Staining: Low Intensity: Weak Hair follicles Staining: Medium Intensity: Moderate Quantity: 75%-25% Sebaceous glands Staining: Low Intensity: Moderate Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25181/53836_B_4_8.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (A549, ASC TERT1, RT4, SK-BR-3, T-47d, U-138 MG, U-87 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000074410-CA12
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Specifications
- Immunogen
- Recombinant extracellular domain of human CA XII expressed in human cell line HEK-293
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human carbonic anhydrase XII (CA XII)
- Species Reactivity
- Human
- Clone
- 14D6
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Inhibition, Functional Assay
- Conjugate
- Unconjugated
- Related Disease
- Tumors
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Inhibition assay: 30 nM-2000 nM
Functional assay: 5 μg/mL-10 μg/mL
Target
- Alternative Names
- CAXII; CA-XII; T18816; HsT18816
- Gene ID
- 771
- UniProt ID
- O43570
- Sequence Similarities
- Belongs to the alpha-carbonic anhydrase family.
- Cellular Localization
- Cell membrane; Membrane
- Protein Refseq
- NP_001209.1; NP_996808.1
- Function
- Reversible hydration of carbon dioxide.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "Clone 14D6"
- CAT
- Product Name
See other products for "CA12"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-086CL | Mouse Anti-CA12 Recombinant Antibody (TAB-086CL) | IP, IF, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1958MZ | Recombinant Mouse Anti-Human CA12 Antibody | WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3987 | Hi-Affi™ Rabbit Anti-CA12 Recombinant Antibody (clone SI47DS) | ELISA, IHC-P | Rabbit IgG |
| MOR-3988 | Hi-Affi™ Rabbit Anti-CA12 Recombinant Antibody (clone SI48DS) | ELISA | Rabbit IgG |
| MOR-3989 | Hi-Affi™ Rabbit Anti-CA12 Recombinant Antibody (clone SI49DS) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY161 | Human Anti-CA12 (clone 14D6) scFv-Fc Chimera | ELISA, WB, FC, Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY289 | Anti-CA12 Immunohistochemistry Kit | IHC | |
| VS-0525-XY901 | Anti-Mouse CA12 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC37 | Recombinant Anti-CA12 Vesicular Antibody, EV Displayed (VS-0425-YC37) | ELISA, FC, Inhib, Cell-uptake |
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