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    Mouse Anti-CA12 Recombinant Antibody (clone 14D6) (CAT#: VS-0323-CJ3)

    This mouse anti-CA12 antibody (clone 14D6) is a product that recognizes the recombinant extracellular domain of Human carbonic anhydrase XII (CA12).

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
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    Other Requirements:
    We require custom production
    • Published Data
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    FC

    Figure 1 The binding of MAb 14D6 with native CA XII localized on the surface of live A498 cells was investigated by flow cytometry.

    Figure 1 The binding of MAb 14D6 with native CA XII localized on the surface of live A498 cells was investigated by flow cytometry.

    Inhib

    Figure 2 Inhibition of CO<sub>2</sub> hydratase activity of CA XIIHEK by the MAb 14D6.

    Figure 2 Inhibition of CO2 hydratase activity of CA XIIHEK by the MAb 14D6.

    WB

    Figure 3 Analysis of the lysates of <em>E. coli</em> expressing overlapping fragments of CA XII with the 14D6-Derived scFv-Fc and anti-human Fc secondary antibody by Western blot.

    Figure 3 Analysis of the lysates of E. coli expressing overlapping fragments of CA XII with the 14D6-Derived scFv-Fc and anti-human Fc secondary antibody by Western blot.

    ELISA

    Figure 4 Apparent dissociation constants (Kd, M) of MAb 14D6, RAb scFv, and RAb scFv-Fc determined by an indirect ELISA.

    Figure 4 Apparent dissociation constants (Kd, M) of MAb 14D6, RAb scFv, and RAb scFv-Fc determined by an indirect ELISA.

    FuncS

    Figure 5 MAb 14D6 showed greater inhibitory activity on A498 cell migration.

    Figure 5 MAb 14D6 showed greater inhibitory activity on A498 cell migration.

    Figure 6 Effect of MAb 14D6 and isotype control (TSPyV MAb 20D3) on human lung adenocarcinoma A498 and A549 spheroids.

    Figure 6 Effect of MAb 14D6 and isotype control (TSPyV MAb 20D3) on human lung adenocarcinoma A498 and A549 spheroids.


    Specifications

    • Immunogen
    • Recombinant extracellular domain of human CA XII expressed in human cell line HEK-293
    • Host Species
    • Mouse
    • Type
    • Mouse IgG1
    • Specificity
    • Human carbonic anhydrase XII (CA XII)
    • Species Reactivity
    • Human
    • Clone
    • 14D6
    • Applications
    • Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Inhibition, Functional Assay
    • Conjugate
    • Unconjugated
    • Related Disease
    • Tumors

    Product Property

    • Purity
    • >95% as determined by SDS-PAGE
    • Buffer
    • PBS
    • Storage
    • Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • Inhibition assay: 30 nM-2000 nM
      Functional assay: 5 μg/mL-10 μg/mL

    Target

    • Alternative Names
    • CAXII; CA-XII; T18816; HsT18816
    • Sequence Similarities
    • Belongs to the alpha-carbonic anhydrase family.
    • Cellular Localization
    • Cell membrane; Membrane
    • Function
    • Reversible hydration of carbon dioxide.

    Recommended Products

    Isotype Control

    Secondary Antibody

    Recommended Dilution Buffer

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "Clone 14D6"

    See other products for "CA12"

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    Please select product type
    Rat Antibody Products IgG Antibody Products Rabbit Monoclonal Antibody Products ScFv-Fc Chimera Products IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry Extracellular Vesicle (EV) Antibody Products
    CAT Product Name Application Type
    TAB-086CL Mouse Anti-CA12 Recombinant Antibody (TAB-086CL) IP, IF, FC Mouse IgG2a

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