Mouse Anti-CA12 Recombinant Antibody (clone 14D6) (CAT#: VS-0323-CJ3)
This mouse anti-CA12 antibody (clone 14D6) is a product that recognizes the recombinant extracellular domain of Human carbonic anhydrase XII (CA12).
Specific Inquiry
FC
Figure 1 The binding of MAb 14D6 with native CA XII localized on the surface of live A498 cells was investigated by flow cytometry.
Inhib
Figure 2 Inhibition of CO2 hydratase activity of CA XIIHEK by the MAb 14D6.
WB
Figure 3 Analysis of the lysates of E. coli expressing overlapping fragments of CA XII with the 14D6-Derived scFv-Fc and anti-human Fc secondary antibody by Western blot.
ELISA
Figure 4 Apparent dissociation constants (Kd, M) of MAb 14D6, RAb scFv, and RAb scFv-Fc determined by an indirect ELISA.
FuncS
Figure 5 MAb 14D6 showed greater inhibitory activity on A498 cell migration.
Figure 6 Effect of MAb 14D6 and isotype control (TSPyV MAb 20D3) on human lung adenocarcinoma A498 and A549 spheroids.
Specifications
- Immunogen
- Recombinant extracellular domain of human CA XII expressed in human cell line HEK-293
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human carbonic anhydrase XII (CA XII)
- Species Reactivity
- Human
- Clone
- 14D6
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Inhibition, Functional Assay
- Conjugate
- Unconjugated
- Related Disease
- Tumors
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Inhibition assay: 30 nM-2000 nM
Functional assay: 5 μg/mL-10 μg/mL
Target
- Alternative Names
- CAXII; CA-XII; T18816; HsT18816
- Gene ID
- 771
- UniProt ID
- O43570
- Sequence Similarities
- Belongs to the alpha-carbonic anhydrase family.
- Cellular Localization
- Cell membrane; Membrane
- Protein Refseq
- NP_001209.1; NP_996808.1
- Function
- Reversible hydration of carbon dioxide.
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CA12"
Rat Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-086CL | Mouse Anti-CA12 Recombinant Antibody (TAB-086CL) | IP, IF, FC | Mouse IgG2a |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1958MZ | Recombinant Mouse Anti-Human CA12 Antibody | WB | Mouse antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3987 | Hi-Affi™ Rabbit Anti-CA12 Recombinant Antibody (clone SI47DS) | ELISA, IHC-P | Rabbit IgG |
| MOR-3988 | Hi-Affi™ Rabbit Anti-CA12 Recombinant Antibody (clone SI48DS) | ELISA | Rabbit IgG |
| MOR-3989 | Hi-Affi™ Rabbit Anti-CA12 Recombinant Antibody (clone SI49DS) | ELISA | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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