Rabbit Anti-CALD1 Recombinant Antibody (clone R01-1G4) (CAT#: VS3-FY1980)

• Immunogen
• Synthetic peptide of human caldesmon/CDM

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human and Rat CALD1

• Species Reactivity
• Human, Rat

• Clone
• R01-1G4

• Applications
• Western Blot, Immunohistochemistry-Paraffin

• MW
• The calculated weight of the target protein is approximately 93 kDa, while the observed molecular weight stands at 80 kDa.

• Related Disease
• Human breast cancer, Mixed Endometrial Stromal And Smooth Muscle Tumor and Kidney Leiomyosarcoma

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/50-1/100

• Alternative Names
• Caldesmon 1; CDM; Testis Secretory Sperm-Binding Protein Li 227n; Caldesmon; H-CAD; L-CAD

• Gene ID
• 800

• UniProt ID
• Q05682

• Sequence Similarities
• Belongs to the caldesmon family.

• Cellular Localization
• Cytoplasm, Cytoskeleton

• Post Translation Modifications
• In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments.
  Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity.
  Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization.
  CDK1-mediated phosphorylation promotes Schwann cell migration during peripheral nerve regeneration ().

• Protein Refseq
• XP_016868139.1; XP_016868141.1; XP_016868143.1; XP_024302710.1; XP_024302711.1

• Function
• Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody