Rabbit Anti-CALD1 Recombinant Antibody (clone R01-1G4)
CAT#: VS3-FY1980
Clone R01-1G4 is a rabbit recombinant monoclonal antibody that attaches to human CALD1. It cross-reacts with rat. R01-1G4 targets CALD1 in WB, IHC-P applications. This caldesmon antibody was verified by western blot analysis of Caldesmon/CDM in Hela, C6 lysates and immunohistochemistry analysis of paraffin-embedded human breast cancer.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & actin filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8066/9_B12_1_selected.jpg
Normal Tissue
Figure 2 High expression in smooth muscle
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8066/21805_B_6_7_val_selected.jpg
Normal Tissue
Figure 3 Low expression in skeletal muscle
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8066/21805_B_3_6_val_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/6/2115_B_7_5.jpg
Normal Tissue
Figure 5 Smooth muscle
Smooth muscle cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/6/2115_B_6_7.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (BJ hTERT+, fHDF/TERT166, U-138 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000122786-CALD1
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WB
Figure 1 Anti-CALD1 Antibody (VS3-FY1980) in WB.
The Caldesmon/CDM expression in Hela, C6 lysates were detected using the anti-Caldesmon by western blot assay.
IHC
Figure 2 Anti-CALD1 Antibody (VS3-FY1980) in IHC.
Immunohistochemistry staining of paraffin-embedded human breast cancer using the anti-Caldesmon/CDM antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide of human caldesmon/CDM
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat CALD1
- Species Reactivity
- Human, Rat
- Clone
- R01-1G4
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 93 kDa, while the observed molecular weight stands at 80 kDa.
- Related Disease
- Human breast cancer, Mixed Endometrial Stromal And Smooth Muscle Tumor and Kidney Leiomyosarcoma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Caldesmon 1; CDM; Testis Secretory Sperm-Binding Protein Li 227n; Caldesmon; H-CAD; L-CAD
- Gene ID
- 800
- UniProt ID
- Q05682
- Sequence Similarities
- Belongs to the caldesmon family.
- Cellular Localization
- Cytoplasm, Cytoskeleton
- Post Translation Modifications
- In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments.
Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity.
Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization.
CDK1-mediated phosphorylation promotes Schwann cell migration during peripheral nerve regeneration ().
- Protein Refseq
- XP_016868139.1; XP_016868141.1; XP_016868143.1; XP_024302710.1; XP_024302711.1
- Function
- Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CALD1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0439 | Hi-Affi™ Rabbit Anti-CALD1 Recombinant Antibody (clone DS439AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK881 | Mouse Anti-CALD1 Recombinant Antibody (VS3-WK881) | WB, IHC | Mouse IgG2b |
| VS3-WK882 | Mouse Anti-CALD1 Recombinant Antibody (clone ABT-CALD) | IHC-P, WB | Mouse IgG2b, κ |
| VS3-WK883 | Rabbit Anti-CALD1 Recombinant Antibody (VS3-WK883) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS3-FY181 | Recombinant Rabbit Anti-CALD1 Antibody (clone R05-3D2) | WB, IHC-P, IP | Rabbit IgG |
| VS3-FY182 | Recombinant Mouse Anti-CALD1 Antibody (clone 9B7-7G7-7A1) | IHC-P | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1289 | AbPlus™ Anti-CALD1 Magnetic Beads (VS-0724-YC1289) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY295 | Anti-CALD1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY939 | Anti-Rat CALD1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY938 | Anti-Human CALD1 Immunohistochemistry Kit | IHC |
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