Rabbit Anti-Casp4 Recombinant Antibody (VS3-FY2603)
CAT#: VS3-FY2603
The anti-Casp4 antibody is specifically engineered to target human caspase 4 protein. It shows capability of cross-reacting with mouse and rat. This monoclonal antibody proves valuable in disease research related to Neuroblastoma and Cowpox.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27588/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27588/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows membrane and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37167/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27588/321_G12_5_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27588/323_G12_3_blue_red_green.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27588/71605_B_9_5.jpg
Normal Tissue
Figure 7 Lung
Macrophages
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27588/71605_A_2_4.jpg
Normal Tissue
Figure 8 Colon
Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27588/71605_A_9_3.jpg
Normal Tissue
Figure 9 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27588/71605_A_7_4.jpg
Normal Tissue
Figure 10 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27588/71605_A_4_6.jpg
Normal Tissue
Figure 11 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27588/71605_A_9_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000196954-CASP4
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Caspase 4 of human, mouse and rat origins
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IP
- Conjugate
- Unconjugated, FITC
- Related Disease
- Neuroblastoma and Cowpox
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunoprecipitation: 1/20
Western Blot: 1/500-1/1000
Target
- Alternative Names
- Caspase 4; Caspase 4, Apoptosis-Related Cysteine Peptidase; Caspase 4, Apoptosis-Related Cysteine Protease; ICE And Ced-3 Homolog 2; Protease TX; ICE(Rel)-II; CASP-4; ICH-2; Mih1; Apoptotic Cysteine Protease Mih1/TX
- Gene ID
- 12363
- UniProt ID
- P70343
- Sequence Similarities
- Belongs to the peptidase C14A family.
- Cellular Localization
- Cytoplasm, cytosol
Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side
Mitochondrion
Inflammasome
Secreted
- Post Translation Modifications
- In response to activation signals, including endoplasmic reticulum stress or treatment with amyloid-beta A4 protein fragments (such as amyloid-beta protein 40), undergoes autoproteolytic cleavage.
(Microbial infection) ADP-riboxanation by S.flexneri OspC3 blocks CASP4 autoprocessing, preventing CASP4 activation and ability to recognize and cleave GSDMD, thereby thwarting the inflammasome/pyroptosis-mediated defense.
Modification sites at PhosphoSitePlus
Modification sites at neXtProt
- Protein Refseq
- XP_011541321.1; XP_054226106.1; NP_001216.1; NP_150649.1
- Function
- Acts as a thiol protease that cleaves a tetrapeptide after an Asp residue at position P1: catalyzes cleavage of CGAS, GSDMD and IL18.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "Casp4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-225-VHH | Recombinant Anti-Mouse Casp4 VHH Single Domain Antibody | WB, IP, ICC, FC, FA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0327CT | Mouse Anti-CASP4 Recombinant Antibody (clone DBT5) | ELISA, WB | Mouse IgG1 |
| VS13-YC128 | Rabbit Anti-Casp4 Recombinant Antibody (VS13-YC128) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0360MZ | Chicken Anti-Mih1/Tx Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1004 | Anti-Casp4 Immunohistochemistry Kit | IHC |
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