Rabbit Anti-CASP9 Recombinant Antibody (clone 2D5) (CAT#: ZG-0581J)

Figure 1 Rabbit Anti-CASP9 Antibody (ZG-0581J) in WB

Western Blot
Positive WB detected in: HL-60 whole cell lysate, LO2 whole cell lysate
All lanes: CASP9 antibody at 1.8μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47, 31, 18, 37 KDa
Observed band size: 47 KDa

Figure 2 Rabbit Anti-CASP9 Antibody (ZG-0581J) in IF

Immunofluorescence staining of HepG2 cells with ZG-0581J at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).

Figure 3 Rabbit Anti-CASP9 Antibody (ZG-0581J) in FC

Overlay histogram showing K562 cells stained with ZG-0581J (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.