Rabbit Anti-CD24 Recombinant Antibody (clone R69-1X-8)
CAT#: VS3-FY2151
This is a rabbit recombinant monoclonal antibody that is specifically developed for the detection of human CD24. The recommended dilutions for western blot (WB) are 1/500-1/1000, and for immunoprecipitation (IP) are 1/50. It is advised to store this antibody at 4°C for short-term use, while for long-term storage, it is recommended to aliquot and store at -20°C to maintain its stability. It is crucial to avoid subjecting the antibody to freeze/thaw cycles to preserve its efficacy.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45879/1529_A2_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/78471/1725_B2_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human esophagus
Immunohistochemical staining of human esophagus shows strong membranous positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/78471/168061_B_5_1_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/78471/168061_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/78471/168061_A_8_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/78471/168061_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/78471/168061_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/78471/168061_A_4_6.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (NTERA-2, RPTEC TERT1, RT4, SK-BR-3)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000272398-CD24
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CD24
- Species Reactivity
- Human
- Clone
- R69-1X-8
- Applications
- Western Blot, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 8kDa, while the observed molecular weight stands at 31-45kDa.
- Related Disease
- Multiple Sclerosis
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/50
Target
- Alternative Names
- CD24 Molecule; CD24 Antigen (Small Cell Lung Carcinoma Cluster 4 Antigen); CD24A; Small Cell Lung Carcinoma Cluster 4 Antigen; Signal Transducer CD24; CD24 Antigen
- Gene ID
- 12484
- UniProt ID
- P25063
- Sequence Similarities
- Belongs to the CD24 family.
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Extensively O-glycosylated. (P25063-CD24_HUMAN)
Glycosylation at Asn36 and Asn52 (NX_P25063 [NX_P25063-1])
Glycosylation from GlyConnect CD24_HUMAN (1086)
Glycosylation from GlyGen (P25063) 3 sites, 13 N-linked glycans (1 site), 1 O-linked glycan (1 site)
- Protein Refseq
- XP_054209946.1; NP_001278666.1; NP_001278667.1; NP_001278668.1; NP_001346013.1
- Function
- May have a pivotal role in cell differentiation of different cell types. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Modulates B-cell activation responses. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-006LC | Mouse Anti-CD24 Recombinant Antibody (TAB-006LC) | FC, FuncS | Mouse IgG2a |
| TAB-006LC-S(P) | Mouse Anti-CD24 Recombinant Antibody; scFv Fragment (TAB-006LC-S(P)) | FC | Mouse scFv |
| TAB-006LC-F(E) | Mouse Anti-CD24 Recombinant Antibody; Fab Fragment (TAB-006LC-F(E)) | FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-007LC | Human Anti-CD24 Recombinant Antibody (TAB-007LC) | FC | Humanized antibody |
| TAB-007LC-S(P) | Human Anti-CD24 Recombinant Antibody; scFv Fragment (TAB-007LC-S(P)) | FC | Humanized scFv |
| TAB-007LC-F(E) | Human Anti-CD24 Recombinant Antibody; Fab Fragment (TAB-007LC-F(E)) | FC | Humanized Fab |
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| MOB-0350MZ | Mouse Anti-CD24 Recombinant Antibody (clone TN4) | ELISA | Mouse IgG1 |
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| VS-0723-WK217 | Human Anti-CD24 Recombinant Antibody (VS-0723-WK217) | ELISA, FC | Chimeric (rabbit/human) IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0434-CN-S(P) | Mouse Anti-CD24 Recombinant Antibody (clone E4); scFv Fragment | WB, ELISA, Neut | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0433-CN-F(E) | Mouse Anti-CD24 Recombinant Antibody Fab Fragment | WB, ELISA, Neut | Mouse Fab |
| HPAB-0434-CN-F(E) | Mouse Anti-CD24 Recombinant Antibody (clone E4); Fab Fragment | WB, ELISA, Neut | Mouse Fab |
| CAT | Product Name | Application | Type |
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| VS-0924-YC23 | Rabbit Anti-CD24 Antibody (VS-0924-YC23) - Cancer Stem Cell Marker | IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| VS-0225-XY27 | CytoStream™ Mouse Anti-CD24 Recombinant Antibody (VS-0225-XY27) | FC | Mouse IgG2a, kappa |
| VS-0225-XY26 | CytoStream™ Mouse Anti-CD24 Recombinant Antibody (VS-0225-XY26) | FC | Mouse IgM, kappa |
| CAT | Product Name | Application | Type |
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| VS-0525-XY1145 | Anti-CD24 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1146 | Anti-Mouse CD24 Immunohistochemistry Kit | IHC |
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