Mouse Anti-CD53 Recombinant Antibody (clone C53-2)
CAT#: VS3-CJ746
This product is a mouse antibody that recognizes human CD53.
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/47216/108620_B_7_5.jpg
Normal Tissue
Figure 2 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/47216/108620_A_7_4.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/47216/108620_A_8_5.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/47216/108620_A_9_8.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (Daudi, HDLM-2, HMC-1, Karpas-707, U-266/70, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143119-CD53
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Specifications
- Immunogen
- Native purified human CD53
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human CD53
- Species Reactivity
- Human
- Clone
- C53-2
- Applications
- IF, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.3.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for one year. Do not freeze.
- Shipping
- Shipped at 4°C
Applications
- Application Notes
- This antibody has been tested for use in Immunofluorescence, Flow Cytometry.
Target
- Alternative Names
- MOX44; TSPAN25
- Gene ID
- 963
- UniProt ID
- P19397
- Sequence Similarities
- Belongs to the tetraspanin (TM4SF) family.
- Cellular Localization
- Cell junction, Cell membrane, Membrane
- Protein Refseq
- NP_000551.1; NP_001035122.1
- Function
- Required for efficient formation of myofibers in regenerating muscle at the level of cell fusion. May be involved in growth regulation in hematopoietic cells (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| VS-0724-YC544 | AbPlus™ Anti-Cd53 Magnetic Beads (VS-0724-YC544) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS-0525-XY1199 | Anti-CD53 Immunohistochemistry Kit | IHC |
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