Mouse Anti-CD68 Recombinant Antibody (clone PT0054) (CAT#: VS3-CJ885)
This product is a mouse antibody that recognizes human CD68.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51/if_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51/207_B3_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51/207_B3_1_blue_red_green.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51/208_B3_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51/208_B3_1_blue_red_green.jpg
Figure 4 Cerebral cortex
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48982/158158_B_8_5.jpg
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48982/158158_B_8_5.jpg
Figure 5 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48982/158158_A_7_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48982/158158_A_7_8.jpg
Figure 6 Spleen
(Cells in red pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48982/158158_B_8_4.jpg
(Cells in red pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48982/158158_B_8_4.jpg
Figure 7 RNA cell line category: Cell line enhanced (ASC diff, U-87 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129226-CD68
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129226-CD68
Figure 7 RNA cell line category: Cell line enhanced (ASC diff, U-87 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129226-CD68
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129226-CD68
Specifications
- Immunogen
- Synthesized peptide derived from human CD68
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CD68
- Species Reactivity
- Human
- Clone
- PT0054
- Applications
- IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunohistochemistry-Parraffin (1:100-1:500).
Target
- Alternative Names
- GP110; LAMP4; SCARD1
- Gene ID
- 968
- UniProt ID
- P34810
- Sequence Similarities
- Belongs to the LAMP family.
- Cellular Localization
- Cell membrane, Endosome, Lysosome, Membrane
- Post Translation Modifications
- N- and O-glycosylated.
- Protein Refseq
- NP_001035148.1; NP_001242.2
- Function
- Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1181z | Mouse Anti-CD68 Recombinant Antibody (clone 24H1) | ELISA, FC, ICC, IF, IHC, WB | Mouse IgG1 |
| FAMAB-1641CQ | Mouse Anti-CD68 Recombinant Antibody (clone FA-11) | IP, FC, IHC | Mouse IgG2a |
| VS3-QX195 | Mouse Anti-CD68 Recombinant Antibody (clone 8A5D12) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-QX196 | Mouse Anti-CD68 Recombinant Antibody (clone 10H7) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-XY393 | Mouse Anti-CD68 Recombinant Antibody (clone 3F7C6) | ELISA, WB, FC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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