Rabbit Anti-CDK2 Recombinant Antibody (clone 2C4) (CAT#: ZG-0611J)
This product is a rabbit antibody that recognizes CDK2.
Specific Inquiry
WB
Figure 1 Rabbit Anti-CDK2 Antibody (ZG-0611J) in WB
Western Blot
Positive WB detected in:Hela whole cell lysate, 293 whole cell lysate(treated with Pervanadate or not)
All lanes:Phospho-CDK2 antibody at 0.8μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34 KDa
Observed band size: 34 KDa
ICC
Figure 2 Rabbit Anti-CDK2 Antibody (ZG-0611J) in ICC
Immunocytochemistry analysis of ZG-0611J diluted at 1:80 and staining in Hela cells(treated with Pervanadate) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IP
Figure 3 Rabbit Anti-CDK2 Antibody (ZG-0611J) in IP
Immunoprecipitating Phospho-CDK2 in Hela whole cell lysate treated with Pervanadate
Lane 1: Rabbit control IgG(1μg)instead of ZG-0611J in Hela whole cell lysate treated with Pervanadate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0611J(3μg)+ Hela whole cell lysate treated with Pervanadate(1mg)
Lane 3: Hela whole cell lysate treated with Pervanadate(20μg)
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-CDK2 (Y15)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CDK2
- Species Reactivity
- Human
- Clone
- 2C4
- Applications
- ELISA, WB, IHC, IP
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunoprecipitation.
WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000
Target
- Alternative Names
- CDKN2; p33(CDK2); cyclin-dependent kinase 2; cdc2-related protein kinase; cell division protein kinase 2; p33 protein kinase
- Gene ID
- 1017
- UniProt ID
- P24941
Related Resources
p53 Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0377CT | Recombinant Mouse anti-Human CDK20 Monoclonal antibody (EML1420) | IHC-P | |
| MRO-1767-CN | Rabbit Anti-CDK2 Polyclonal Antibody (MRO-1767-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0043U | Rabbit Anti-CDK2 Recombinant Antibody (clone 6A5) | ELISA, WB, IHC | Rabbit IgG |
| VS3-QX214 | Mouse Anti-CDK2 Recombinant Antibody (clone 1A6) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-QX215 | Mouse Anti-CDK2 Recombinant Antibody (clone 5H2-H5-F8) | WB | Mouse IgG2b |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0627 | Hi-Affi™ Rabbit Anti-CDK2 Recombinant Antibody (clone DS627AB) | ICC, IHC-P, IP, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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