Rabbit Anti-CDK2 Recombinant Antibody (clone 6A5)
CAT#: ZG-0043U
The purified Rabbit Anti-CDK2 Recombinant Antibody (clone 6A5) displayed high specificity against the CDK2 protein of human and mouse.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-CDK2 Antibody (ZG-0043U) in WB
Positive WB detected in: K562 whole cell lysate, HEK293 whole cell lysate, NIH/3T3 whole cell lysate, Hela whole cell lysate, HL-60 whole cell lysate, A549 whole cell lysate. All lanes: CDK2 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34, 31 kDa
Observed band size: 34 kDa
IHC
Figure 2 Rabbit Anti-CDK2 Antibody (ZG-0043U) in IHC
IHC image of ZG-0043U diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Normal Tissue
Figure 1 Testis
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13115/32765_A_6_6.jpg
Normal Tissue
Figure 2 Colon
Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13115/32765_A_8_3.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13115/32765_A_7_5.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Pachytene spermatocytes Staining: Low Intensity: Moderate Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13115/32765_A_6_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13115/32765_A_7_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000123374-CDK2
Normal Tissue
Figure 2 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13115/32765_A_7_8.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000123374-CDK2
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Specifications
- Immunogen
- A synthesized peptide derived from human Cdk2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CDK2
- Species Reactivity
- Human, Mouse
- Clone
- 6A5
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Breast Cancer and Retinoblastoma
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# ZG-0043U, RRID: AB_3714421)
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Related Signaling Pathways
p53 Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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