Rabbit Anti-CDK2 Recombinant Antibody (clone 6A5) (CAT#: ZG-0043U)
The purified Rabbit Anti-CDK2 Recombinant Antibody (clone 6A5) displayed high specificity against the CDK2 protein of human and mouse.
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Custom Production
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WB
Figure 1 Rabbit Anti-CDK2 Antibody (ZG-0043U) in WB
Positive WB detected in: K562 whole cell lysate, HEK293 whole cell lysate, NIH/3T3 whole cell lysate, Hela whole cell lysate, HL-60 whole cell lysate, A549 whole cell lysate. All lanes: CDK2 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34, 31 kDa
Observed band size: 34 kDa
IHC
Figure 2 Rabbit Anti-CDK2 Antibody (ZG-0043U) in IHC
IHC image of ZG-0043U diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human Cdk2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CDK2
- Species Reactivity
- Human, Mouse
- Clone
- 6A5
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Breast Cancer and Retinoblastoma
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- Cyclin-dependent kinase 2; EC 2.7.11.22; Cell division protein kinase 2; p33 protein kinase; CDK2; CDKN2
- Gene ID
- 1017
- UniProt ID
- P24941
Related Resources
p53 Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1714z | Mouse Anti-CDK2 Recombinant Antibody (clone 21A9) | WB, FC, ICC, IF | Mouse IgG1 |
| MOB-0377CT | Recombinant Mouse anti-Human CDK20 Monoclonal antibody (EML1420) | IHC-P | |
| VS3-CJ813 | Mouse Anti-CDK2 Recombinant Antibody (VS3-CJ813) | WB | Mouse IgG1, κ |
| VS3-WK2268 | Rabbit Anti-CDK2 (pT14) Recombinant Antibody (VS3-WK2268) | WB, IHC, IP | Rabbit IgG |
| VS13-YC197 | Rabbit Anti-CDK2 Recombinant Antibody (VS13-YC197) | WB, ICC, IF, IHC-P, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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