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CDKN1B
Rabbit Anti-CDKN1B (pS10) Recombinant Antibody (clone R05-4F1)

Rabbit Anti-CDKN1B (pS10) Recombinant Antibody (clone R05-4F1) (CAT#: VS3-WK2006)

Name: Rabbit Anti-CDKN1B (pS10) Recombinant Antibody (clone R05-4F1)
SKU: VS3-WK2006
Rating: 5 (1 reviews)

This product is a rabbit recombinant antibody that is specific to phosphorylated CDKN1B. The target of this antibody is phospho-CDKN1B (Ser10). The clone R05-4F1 can be used in WB analysis at a dilution of 1/500-1/1000, IP analysis at a dilution of 1/20, and IHC staining at a dilution of 1/50-1/100. The molecular weight of CDKN1B (pSer10) observed in WB experiments using this antibody is 27 kDa.

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1 Rabbit Anti-CDKN1B (pSer10) Recombinant Antibody (clone R05-4F1) in WB.

Figure 1 Rabbit Anti-CDKN1B (pSer10) Recombinant Antibody (clone R05-4F1) in WB.

Western blot analysis of phospho-p27 KIP 1 (Ser10) in C6 lysates using anti-CDKN1B (pSer10) antibody, Cat# VS3-WK2006.

IHC-P

Figure 2 Rabbit Anti-CDKN1B (pSer10) Recombinant Antibody (clone R05-4F1) in IHC-P.

Figure 2 Rabbit Anti-CDKN1B (pSer10) Recombinant Antibody (clone R05-4F1) in IHC-P.

Immunohistochemistry analysis of paraffin-embedded human breast cancer using anti-CDKN1B (pSer10) antibody, Cat# VS3-WK2006.

Subcellular Location

Figure 1 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/59086/if_selected.jpg

Normal Tissue

Figure 2 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21888/48637_B_7_5.jpg

Figure 3 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21888/48637_A_8_3.jpg

Figure 4 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21888/48637_A_9_4.jpg

Figure 5 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Cells in tubules Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21888/48637_A_8_5.jpg

Figure 6 Testis
(Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21888/48637_A_5_6.jpg

Figure 7 Lymph node
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21888/48637_A_7_8.jpg

RNA Expression

Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111276-CDKN1B

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
A synthetic phosphopeptide corresponding to residues Ser10 of human p27 KIP 1

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Phospho-Ser10 of human, mouse and rat CDKN1B

Species Reactivity
Human, Mouse, Rat

Clone
R05-4F1

Applications
WB, IHC-P, IP

Conjugate
Unconjugated

Product Property

Clonality
Monoclonal

Purification
Protein A affinity purified

Purity
>95% as determined by SDS-PAGE

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
WB: 1/500-1/1000
IHC: 1/50-1/100
IP: 1/20

Target

Alternative Names
Cyclin Dependent Kinase Inhibitor 1B; Cyclin-Dependent Kinase Inhibitor 1B (P27, Kip1); P27KIP1; KIP1; Cyclin-Dependent Kinase Inhibitor P27; Cyclin-Dependent Kinase Inhibitor 1B; CDKN4; MEN1B; MEN4

Gene ID
1027

UniProt ID
P46527

Long Name
Cyclin Dependent Kinase Inhibitor 1B

Isotype Control

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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CAT	Product Name	Application	Type
MOB-2451z	Mouse Anti-CDKN1B Recombinant Antibody (clone 33H2)	WB, ELISA, ICC, IF	Mouse IgG1
MOB-1429CT	Recombinant Mouse anti-Human CDKN1B Monoclonal antibody (EML014)	ICC/IF, IHC-P, WB, sELISA
ZG-0798F	Mouse Anti-CDKN1B Recombinant Antibody (clone DCS72)	WB, IP, IHC-P	Mouse IgG1
VS3-QX219	Mouse Anti-CDKN1B Recombinant Antibody (clone 5B4-E6-E11-A8)	WB	Mouse IgG1
VS3-QX220	Mouse Anti-CDKN1B Recombinant Antibody (clone 1373CT407.103.103)	WB, IHC	Mouse IgG1, κ

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