Mouse Anti-NHP CDKN1B Recombinant Antibody (clone SPM348)
CAT#: VS-1024-XY359
This mouse-derived antibody, directed against CDKN1B, is designed to recognize a broad spectrum of species, including human, mouse, non-human primate, and rat. Generated using a purified GST-p27 fusion protein as the immunogen, it showcases exceptional utility in Immunohistochemistry (IHC) and Immunocytochemistry (ICC) applications. This antibody is instrumental in elucidating the regulatory mechanisms governing cell cycle progression and associated pathologies. By integrating this robust tool into your research, you can gain deeper insights into the roles of CDKN1B in cellular proliferation and differentiation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59086/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21888/48637_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21888/48637_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21888/48637_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Cells in tubules Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21888/48637_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21888/48637_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21888/48637_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111276-CDKN1B
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Specifications
- Immunogen
- Purified GST-p27 fusion protein
- Host Species
- Mouse
- Type
- Mouse IgG1, kappa
- Specificity
- CDKN1B
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- SPM348
- Applications
- IHC, ICC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 0.05% BSA
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- IHC: 1-2 µg/mL, ICC: 2-4 µg/mL
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CDKN1B"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0115MZ | Chicken Anti-CDKN1B Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0640 | Hi-Affi™ Rabbit Anti-CDKN1B Recombinant Antibody (clone DS640AB) | IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX219 | Mouse Anti-CDKN1B Recombinant Antibody (clone 5B4-E6-E11-A8) | WB | Mouse IgG1 |
| VS3-QX220 | Mouse Anti-CDKN1B Recombinant Antibody (clone 1373CT407.103.103) | WB, IHC | Mouse IgG1, κ |
| VS3-XY479 | Mouse Anti-CDKN1B Recombinant Antibody (clone 3D8) | ELISA, WB | Mouse IgG1 |
| VS3-FY353 | Recombinant Mouse Anti-CDKN1B Antibody (clone 5H1-8C7-4C7) | IHC-P | Mouse IgG1 |
| VS3-WK2006 | Rabbit Anti-CDKN1B (pS10) Recombinant Antibody (clone R05-4F1) | WB, IHC-P, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY412 | Anti-CDKN1B Immunohistochemistry Kit | IHC | |
| VS-0525-XY1335 | Anti-Mouse CDKN1B Immunohistochemistry Kit | IHC | |
| VS-0525-XY1334 | Anti-Human CDKN1B Immunohistochemistry Kit | IHC | |
| VS-0525-XY1336 | Anti-Canine CDKN1B Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC202 | CytoStream™ Rabbit Anti-CDKN1B Recombinant Antibody (VS13-YC202) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
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