Mouse Anti-CDKN1B Recombinant Antibody (MOB-0150F)
CAT#: MOB-0150F
This product is a mouse antibody that recognizes CDKN1B.
Tested Data
Gene Expression
IHC
Figure 1 Mouse Anti-CDKN1B Recombinant Antibody (MOB-0150F) in IHC
Immunohistochemical analysis of paraffin-embedded tonsils-high magnification. 1. The antibody is diluted 1:200 (overnight at 4°C). 2. Use pH8.0 TRISEDTA for antigen retrieval. 3. Dilute the secondary antibody at 1:200 (room temperature, 30min).
IHC
Figure 2 Mouse Anti-CDKN1B Recombinant Antibody (MOB-0150F) in IHC
Immunohistochemical analysis of paraffin-embedded tonsils. 1. The antibody is diluted 1:200 (overnight at 4°C). 2. Use pH8.0 TRIS-EDTA for antigen retrieval. 3. Dilute the secondary antibody at 1:200 (room temperature, 30min).
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Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59086/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21888/48637_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21888/48637_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21888/48637_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Cells in tubules Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21888/48637_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21888/48637_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21888/48637_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111276-CDKN1B
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Specifications
- Immunogen
- Synthesized peptide derived from human p27Kip1
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CDKN1B
- Species Reactivity
- Human, Mouse
- Applications
- IHC-P, IF
- Conjugate
- Unconjugated
Product Property
- Format
- Liquid
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunohistologyp-Paraffin Sections, Immunofluorescence.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| BRD-0115MZ | Chicken Anti-CDKN1B Polyclonal IgY | WB | Chicken antibody |
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| CAT | Product Name | Application | Type |
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| MRO-1143-CN | Rabbit Anti-CDKN1B Recombinant Antibody (clone CBACN-416) | WB, IF, IHC, IP, FC | Rabbit IgG |
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| VS3-CJ975 | Rabbit Anti-CDKN1B Recombinant Antibody (VS3-CJ975) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY359 | Mouse Anti-NHP CDKN1B Recombinant Antibody (clone SPM348) | IHC, ICC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
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| VS-0325-XY412 | Anti-CDKN1B Immunohistochemistry Kit | IHC | |
| VS-0525-XY1335 | Anti-Mouse CDKN1B Immunohistochemistry Kit | IHC | |
| VS-0525-XY1334 | Anti-Human CDKN1B Immunohistochemistry Kit | IHC | |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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