Mouse Anti-CDKN1B Recombinant Antibody (clone 1A7) (CAT#: VS3-WK1591)

• Immunogen
• Synthesized peptide derived from human P27 Kip1

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human CDKN1B

• Species Reactivity
• Human, Mouse, Rat

• Clone
• 1A7

• Applications
• IHC-P, WB

• Conjugate
• Unconjugated

• Clonality
• Monoclonal

• Purification
• Protein G affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Aggregation
• < 5%

• Format
• Liquid

• Concentration
• 1 mg/mL

• Buffer
• PBS, 0.5% BSA, 50% Glycerol

• Preservative
• 0.02% Sodium Azide

• Storage
• Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• IHC-P: 1/50-1/200
  WB: 1/1000-1/2000

• Alternative Names
• KIP1; MEN4; CDKN4; MEN1B; P27KIP1

• Gene ID
• 1027

• UniProt ID
• P45627

• Long Name
• Cyclin dependent kinase inhibitor 1B

• Sequence Similarities
• Belongs to the CDI family.

• Cellular Localization
• Cytoplasm, Endosome, Nucleus

• Post Translation Modifications
• Phosphorylated; phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G0-G1 phase and leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC and in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK1 and CDK2 leads to protein ubiquitination and proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 and Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF.
  Ubiquitinated; in the cytoplasm by the KPC complex (composed of RNF123/KPC1 and UBAC1/KPC2) and, in the nucleus, by SCF(SKP2). The latter requires prior phosphorylation on Thr-187. Ubiquitinated; by a TRIM21-containing SCF(SKP2)-like complex; leads to its degradation.
  Subject to degradation in the lysosome. Interaction with SNX6 promotes lysosomal degradation (By similarity).

• Protein Refseq
• NP_004055.1

• Function
• Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA.
  Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Secondary Antibody

• 2RD-A1514-YJ
• Sheep Anti-Mouse IgG-heavy and light chain Secondary antibody

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

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