Mouse Anti-CDKN2A Recombinant Antibody (clone R15-A)
CAT#: ZG-0394U
Mouse anti-CDKN2A antibody (clone R15-A) displayed high specificity against the CDKN2A protein of Human.
Tested Data
Gene Expression
IHC-P
Figure 1 Mouse Anti-CDKN2A Antibody (ZG-0394U) in IHC-P
High grade squamous intraepithelial lesion of the uterine cervix stained with mouse anti-p16 (clone R15-A) monoclonal antibody, shows significant nuclear and cytoplasmic positivity of target cells. Formalin fixed, paraffin embedded human tissues (4 μm sections) stained.
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Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47838/979_F9_4_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/93/ihc_selected.jpg
Normal Tissue
Figure 3 Tonsil
High expression in tonsil
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/445/2553_A_4_8_val_selected.jpg
Normal Tissue
Figure 4 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong positivity in a subset of cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18232/ihc_selected.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/445/2553_A_8_8.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/445/2553_A_8_5.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (CAPAN-2, HEK93, HeLa, SK-MEL-30)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147889-CDKN2A
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Specifications
- Immunogen
- Human p16 protein
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CDKN2A
- Species Reactivity
- Human
- Clone
- R15-A
- Applications
- IHC-P
- Conjugate
- Unconjugated
- Related Disease
- Melanoma-Pancreatic Cancer Syndrome and Melanoma, Cutaneous Malignant 2.
Product Property
- Purification
- Affinity Purified
- Buffer
- 20 mM Tris-HCl, pH 8.0, 20 mg/ml BSA and 0.05% sodium azide
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunohistochemistry-Paraffin. Specific conditions should be optimized by the end user.
IHC-P: 1:100
Target
- Alternative Names
- ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A; cyclin-dependent kinase inhibitor 2A; CDK4 inhibitor p16-INK4; alternative reading frame; cell cycle negative regulator beta; cyclin-dependent kinase 4 inhibitor A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4); multiple tumor suppressor 1
- Gene ID
- 1029
- UniProt ID
- P42771
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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