CytoStream™ Rabbit Anti-CEBPA Polyclonal Antibody (VS13-YC206)
CAT#: VS13-YC206
The CytoStream™ Rabbit Anti-CEBPA Polyclonal Antibody is specific for human and rat CEBPA. CEBPA, a transcription factor, modulates gene expression affecting cell cycle and weight regulation, linked to acute myeloid leukemia. The anti-CEBPA antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1 μg per 1x 10⁶ cells).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52734/1549_E6_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67937/1528_A1_1_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65037/ihc_selected.jpg
Normal Tissue
Figure 4 Pancreas
Exocrine glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65037/150497_A_1_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65037/150497_A_9_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (BEWO, Hep G2, NB-4, THP-1, U-937)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000245848-CEBPA
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Specifications
- Immunogen
- Recombinant human CEBP-alpha (aa 51-358) conjugated with KLH.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat CEBPA
- Species Reactivity
- Human, Rat, Mouse (Predicted), Dog (Predicted), Pig (Predicted), Cow (Predicted), Rabbit (Predicted), Sheep (Predicted), Goat (Predicted)
- Applications
- WB, ELISA, IHC-P, IHC-Fr, FC, ICC, IF
- Conjugate
- NONE
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01× TBS (pH 7.4) containing 50% Glycerol and 1% BSA.
- Preservative
- 0.03% Proclin 300
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
ELISA: 1/5000-1/10000
Immunohistochemistry-paraffin (IHC-P): 1/100-1/500
Immunohistochemistry-frozen (IHC-Fr): 1/100-1/500
Flow cytometry (FC): 1 μg per 1x 10⁶ cells
Immunocytochemistry (ICC): 1/100
Immunofluorescence (IF): 1/100-1/500
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CEBPA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3995z | Mouse Anti-CEBPA Recombinant Antibody (clone 26G6) | WB, ELISA, FC, IF, IHC | Mouse IgG1 |
| MRO-0350-CN | Rabbit Anti-CEBPA Recombinant Antibody (clone CBACN-145) | WB, IP, FC | Rabbit IgG |
| VS3-WK59 | Mouse Anti-CEBPA Recombinant Antibody (clone E8-B7) | WB, ELISA | Mouse IgG1 |
| VS3-XY490 | Mouse Anti-CEBPA Recombinant Antibody (clone 4E10) | ELISA, WB, FC | Mouse IgG1 |
| VS3-XY491 | Mouse Anti-CEBPA Recombinant Antibody (clone 5B7) | ELISA, WB, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0653 | Hi-Affi™ Rabbit Anti-CEBPA Recombinant Antibody (clone DS653AB) | FC, ICC, IF, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC401 | AbPlus™ Anti-CEBPA Magnetic Beads (VS-0724-YC401) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1361 | Anti-CEBPA Immunohistochemistry Kit | IHC |
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