CytoStream™ Rabbit Anti-CEBPA Polyclonal Antibody (VS13-YC206) (CAT#: VS13-YC206)
The CytoStream™ Rabbit Anti-CEBPA Polyclonal Antibody is specific for human and rat CEBPA. CEBPA, a transcription factor, modulates gene expression affecting cell cycle and weight regulation, linked to acute myeloid leukemia. The anti-CEBPA antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1 μg per 1x 10⁶ cells).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line CACO-2
(Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52734/1549_E6_1_selected.jpg
(Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52734/1549_E6_1_selected.jpg
Figure 2 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67937/1528_A1_1_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67937/1528_A1_1_selected.jpg
Figure 3 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65037/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65037/ihc_selected.jpg
Figure 4 Pancreas
(Exocrine glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65037/150497_A_1_3.jpg
(Exocrine glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65037/150497_A_1_3.jpg
Figure 5 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65037/150497_A_9_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65037/150497_A_9_5.jpg
Figure 6 RNA cell line category: Cell line enhanced (BEWO, Hep G2, NB-4, THP-1, U-937)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000245848-CEBPA
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000245848-CEBPA
Specifications
- Immunogen
- Recombinant human CEBP-alpha (aa 51-358) conjugated with KLH.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat CEBPA
- Species Reactivity
- Human, Rat, Mouse (Predicted), Dog (Predicted), Pig (Predicted), Cow (Predicted), Rabbit (Predicted), Sheep (Predicted), Goat (Predicted)
- Applications
- WB, ELISA, IHC-P, IHC-Fr, FC, ICC, IF
- Conjugate
- NONE
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01× TBS (pH 7.4) containing 50% Glycerol and 1% BSA.
- Preservative
- 0.03% Proclin 300
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
ELISA: 1/5000-1/10000
Immunohistochemistry-paraffin (IHC-P): 1/100-1/500
Immunohistochemistry-frozen (IHC-Fr): 1/100-1/500
Flow cytometry (FC): 1 μg per 1x 10⁶ cells
Immunocytochemistry (ICC): 1/100
Immunofluorescence (IF): 1/100-1/500
Target
- Alternative Names
- CCAAT/Enhancer Binding Protein Alpha; CCAAT/Enhancer Binding Protein (C/EBP), Alpha; CEBP; CCAAT/Enhancer-Binding Protein Alpha; C/EBP-Alpha; C/EBP Alpha;
- Gene ID
- 1050
- UniProt ID
- P49715
- Long Name
- CCAAT/Enhancer Binding Protein Alpha
- Cellular Localization
- Nucleus
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3995z | Mouse Anti-CEBPA Recombinant Antibody (clone 26G6) | WB, ELISA, FC, IF, IHC | Mouse IgG1 |
| MRO-0350-CN | Rabbit Anti-CEBPA Recombinant Antibody (clone CBACN-145) | WB, IP, FC | Rabbit IgG |
| VS3-WK59 | Mouse Anti-CEBPA Recombinant Antibody (clone E8-B7) | WB, ELISA | Mouse IgG1 |
| VS3-XY490 | Mouse Anti-CEBPA Recombinant Antibody (clone 4E10) | ELISA, WB, FC | Mouse IgG1 |
| VS3-XY491 | Mouse Anti-CEBPA Recombinant Antibody (clone 5B7) | ELISA, WB, FC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
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