CytoStream™ Rabbit Anti-CEBPA Polyclonal Antibody (VS13-YC206) (CAT#: VS13-YC206)
The CytoStream™ Rabbit Anti-CEBPA Polyclonal Antibody is specific for human and rat CEBPA. CEBPA, a transcription factor, modulates gene expression affecting cell cycle and weight regulation, linked to acute myeloid leukemia. The anti-CEBPA antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1 μg per 1x 10⁶ cells).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line CACO-2
(Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52734/1549_E6_1_selected.jpg
(Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52734/1549_E6_1_selected.jpg
Figure 2 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67937/1528_A1_1_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67937/1528_A1_1_selected.jpg
Figure 3 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65037/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65037/ihc_selected.jpg
Figure 4 Pancreas
(Exocrine glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65037/150497_A_1_3.jpg
(Exocrine glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65037/150497_A_1_3.jpg
Figure 5 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65037/150497_A_9_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65037/150497_A_9_5.jpg
Figure 6 RNA cell line category: Cell line enhanced (BEWO, Hep G2, NB-4, THP-1, U-937)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000245848-CEBPA
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000245848-CEBPA
Specifications
- Immunogen
- Recombinant human CEBP-alpha (aa 51-358) conjugated with KLH.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat CEBPA
- Species Reactivity
- Human, Rat, Mouse (Predicted), Dog (Predicted), Pig (Predicted), Cow (Predicted), Rabbit (Predicted), Sheep (Predicted), Goat (Predicted)
- Applications
- WB, ELISA, IHC-P, IHC-Fr, FC, ICC, IF
- Conjugate
- NONE
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01× TBS (pH 7.4) containing 50% Glycerol and 1% BSA.
- Preservative
- 0.03% Proclin 300
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
ELISA: 1/5000-1/10000
Immunohistochemistry-paraffin (IHC-P): 1/100-1/500
Immunohistochemistry-frozen (IHC-Fr): 1/100-1/500
Flow cytometry (FC): 1 μg per 1x 10⁶ cells
Immunocytochemistry (ICC): 1/100
Immunofluorescence (IF): 1/100-1/500
Target
- Alternative Names
- CCAAT/Enhancer Binding Protein Alpha; CCAAT/Enhancer Binding Protein (C/EBP), Alpha; CEBP; CCAAT/Enhancer-Binding Protein Alpha; C/EBP-Alpha; C/EBP Alpha;
- Gene ID
- 1050
- UniProt ID
- P49715
- Long Name
- CCAAT/Enhancer Binding Protein Alpha
- Cellular Localization
- Nucleus
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0653 | Hi-Affi™ Rabbit Anti-CEBPA Recombinant Antibody (clone DS653AB) | FC, ICC, IF, IP, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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