Mouse Anti-CFH Recombinant Antibody (clone OX23)
CAT#: FAMAB-1105CQ
This product is a recombinant mouse antibody that recognizes CFH. The antibody was purified by affinity chromatography. It can be used in IP, WB, Block, ELISA.
Published Data
Gene Expression
IF
Figure 1 Endogenous CFH associates with GAGs in Bruch’s membrane and RPE within human macula.
Endogenous CFH was detected (using OX23 monoclonal antibodies and the appropriate Alexa Fluor 488-labeled secondary antibody) in human eye sections from five heterozygous individuals either untreated or following treatment with Heparinase and chondroitinase B.
Clark, S. J., Perveen, R., Hakobyan, S., Morgan, B. P., Sim, R. B., Bishop, P. N., & Day, A. J. (2010). Impaired binding of the age-related macular degeneration-associated complement factor H 402H allotype to Bruch's membrane in human retina. Journal of Biological Chemistry, 285(39), 30192-30202.
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Normal Tissue
Figure 1 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong plasma positivity.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49176/ihc_selected.jpg
Normal Tissue
Figure 2 IHC staining of human ovary
Immunohistochemical staining of human ovary shows distinct positivity in plasma.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53326/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows distinct positivity in plasma.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16385/ihc_selected.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16385/37049_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16385/37049_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16385/37049_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HHSteC, HSkMC, hTERT-RPE1, RT4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000000971-CFH
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Specifications
- Immunogen
- Native protein purified from plasma
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Factor H
- Species Reactivity
- Primate, Human, Mouse
- Clone
- OX23
- Applications
- IF
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Immunofluorescence.
Target
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comprehensive guidelines
The comprehensive instructions that came with this product have been very beneficial in assuring correct use and optimizing its efficacy.
Reliable performance
Every batch of this product has continuously delivered exceptional performance, which is essential for our long-term projects.
Detailed protocols provided
The antibody was provided with complete methods that really aided in directing our investigation. They assured that we received the best results by reviewing a number of applications and troubleshooting strategies.
Q&As
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Do I need to filter the antibody solution?
A: Filtration might be required if the solution seems turbid or as instructed by the manufacturer. Employ filters with low protein binding to minimize antibody loss.
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Can antibodies be stored in buffer solutions?
A: Antibodies are usually kept in buffer solutions containing BSA or glycerol. Examine the supplier's precise recommendations.
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How long can an antibody remain stable at room temperature?
A: Antibodies should not be stored at room temperature for an extended duration. Handling for brief periods, such as thirty to sixty minutes, is frequently acceptable.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone OX23"
- CAT
- Product Name
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Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1578z | Mouse Anti-CFH Recombinant Antibody (clone 31G10) | ELISA, IHC, IP | Mouse IgG2b, κ |
| MOB-1341MZ | Recombinant Mouse Anti-Human CFH Antibody (clone 74H6) | ELISA, IP | Mouse antibody |
| HPAB-M0038-YC | Human Anti-CFH Recombinant Antibody (HPAB-M0038-YC) | ELISA | Human IgG |
| HPAB-0515YY | Human Anti-CFH Recombinant Antibody (HPAB-0515YY) | ELISA, FC | Human IgG1, κ |
| VS13-YC214 | Rabbit Anti-CFH Recombinant Antibody (VS13-YC214) | WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-063MZ | Mouse Anti-CFH Recombinant Antibody (TAB-063MZ) | ELISA, Inhib, IF | Mouse IgG1 |
| TAB-064MZ | Mouse Anti-CFH Recombinant Antibody (TAB-064MZ) | ELISA, Inhib | Mouse IgG1 |
| TAB-063MZ-S(P) | Mouse Anti-CFH Recombinant Antibody; scFv Fragment (TAB-063MZ-S(P)) | ELISA | Mouse scFv |
| TAB-063MZ-F(E) | Mouse Anti-CFH Recombinant Antibody; Fab Fragment (TAB-063MZ-F(E)) | ELISA | Mouse Fab |
| TAB-064MZ-F(E) | Mouse Anti-CFH Recombinant Antibody; Fab Fragment (TAB-064MZ-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0674 | Hi-Affi™ Rabbit Anti-CFH Recombinant Antibody (clone DS674AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC4399 | PE-A*01:01/Human CFH (CSDDGFWSK) MHC Tetramer | FCM | |
| MHC-LC4400 | PE-A*11:01/Human CFH (CSDDGFWSK) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0038-YC-F(E) | Human Anti-CFH Recombinant Antibody Fab Fragment (HPAB-M0038-YC-F(E)) | ELISA | Human Fab |
| HPAB-N0047-YC-F(E) | Human Anti-CFH Recombinant Antibody (clone TRN-1006); Fab Fragment | ELISA, Inhib | Human Fab |
| HPAB-0512-YJ-F(E) | Human Anti-CFH Recombinant Antibody; Fab Fragment (HPAB-0512-YJ-F(E)) | ELISA, DB, FuncS | Human Fab |
| HPAB-0515YY-F(E) | Human Anti-CFH Recombinant Antibody; Fab Fragment (HPAB-0515YY-F(E)) | ELISA, FC | Human Fab |
| HPAB-0520YY-F(E) | Human Anti-CFH Recombinant Antibody; Fab Fragment (HPAB-0520YY-F(E)) | ELISA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0047-YC-S(P) | Human Anti-CFH Recombinant Antibody (clone TRN-1006); scFv Fragment | ELISA, Inhib | Human scFv |
| HPAB-0512-YJ-S(P) | Human Anti-CFH Recombinant Antibody; scFv Fragment (HPAB-0512-YJ-S(P)) | ELISA, DB, FuncS | Human scFv |
| HPAB-0515YY-S(P) | Human Anti-CFH Recombinant Antibody; scFv Fragment (HPAB-0515YY-S(P)) | ELISA, FC | Human scFv |
| HPAB-0520YY-S(P) | Human Anti-CFH Recombinant Antibody; scFv Fragment (HPAB-0520YY-S(P)) | ELISA, FC | Human scFv |
| HPAB-0521YY-S(P) | Human Anti-CFH Recombinant Antibody; scFv Fragment (HPAB-0521YY-S(P)) | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY432 | Anti-CFH Immunohistochemistry Kit | IHC | |
| VS-0525-XY1394 | Anti-Human CFH Immunohistochemistry Kit | IHC |
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