Rabbit Anti-CHD3 Recombinant Antibody (clone R05-6G3) (CAT#: VS3-XY3313)
This product is a recombinant rabbit antibody targeting the CHD3 (Chromodomain helicase DNA binding protein 3), which involves in transcriptional repression as part of the NuRD complex. It recognizes and binds to human CDH3. It also has cross-reactivity against mouse and rat CHD3. It has been determined using Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1674_H9_32_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1674_H9_32_selected.jpg
Figure 2 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows distinct nuclear positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/ihc_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows distinct nuclear positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/ihc_selected.jpg
Figure 3 IF staining of human cell line REH
(Immunofluorescent staining of human cell line REH shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1676_H9_2_red_green.jpg
(Immunofluorescent staining of human cell line REH shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1676_H9_2_red_green.jpg
Figure 4 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1712_C4_3_cr5804e5a4d2793_red_green.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1712_C4_3_cr5804e5a4d2793_red_green.jpg
Figure 5 Cerebral cortex
(Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_B_8_5.jpg
(Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_B_8_5.jpg
Figure 6 Colon
(Endocrine cells
Staining:Medium
Intensity: Moderate
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Fibroblasts
Staining:High
Intensity: Strong
Mucosal lymphoid cells
Staining:High
Intensity: Strong
Quantity:>75%
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_A_7_3.jpg
(Endocrine cells
Staining:Medium
Intensity: Moderate
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Fibroblasts
Staining:High
Intensity: Strong
Mucosal lymphoid cells
Staining:High
Intensity: Strong
Quantity:>75%
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_A_7_3.jpg
Figure 7 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_A_6_6.jpg
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_A_6_6.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170004-CHD3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170004-CHD3
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CHD3
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-6G3
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Snijders Blok-Campeau Syndrome; Stuttering
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- ZFH; Mi-2a; SNIBCPS; Mi2-ALPHA
- Gene ID
- 1107
- UniProt ID
- Q12873
- Long Name
- Chromodomain helicase DNA binding protein 3
- Cellular Localization
- Nucleus, PML body, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
- Post Translation Modifications
- Sumoylation at Lys-1971 results in dissociation from chromatin and suppression of transcriptional repression. (Q12873-CHD3_HUMAN)
Modification sites at PhosphoSitePlus (Q12873)
Modification sites at neXtProt (NX_Q12873)
Glycosylation from GlyGen (Q12873) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_005256484.1; XP_005256485.1
- Function
- ATP-dependent helicase that binds and distorts nucleosomal DNA. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin.
Involved in transcriptional repression as part of the NuRD complex.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-299 | Human Anti-CHD3 Recombinant Antibody (clone D404) | ELISA, IP-MS | Human IgG |
| MOB-0486CT | Recombinant Mouse anti-Human CHD3 Monoclonal antibody (EML044) | IHC-P, WB | |
| ZG-0416F | Mouse Anti-CHD3 Recombinant Antibody (ZG-0416F) | WB, ICC | Mouse IgG |
| VS4-WK106 | Mouse Anti-CHD3 Recombinant Antibody (VS4-WK106) | ELISA, WB, ICC | Mouse IgG1 |
| VS7-HM703 | Mouse Anti-CHD3 Recombinant Antibody (clone 2G4) | WB, IHC, ICC, FCM | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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