Rabbit Anti-CHD3 Recombinant Antibody (clone R05-6G3)
CAT#: VS3-XY3313
This product is a recombinant rabbit antibody targeting the CHD3 (Chromodomain helicase DNA binding protein 3), which involves in transcriptional repression as part of the NuRD complex. It recognizes and binds to human CDH3. It also has cross-reactivity against mouse and rat CHD3. It has been determined using Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1674_H9_32_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows distinct nuclear positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line REH
Immunofluorescent staining of human cell line REH shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1676_H9_2_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1712_C4_3_cr5804e5a4d2793_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endocrine cells
Staining:Medium
Intensity: Moderate
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Fibroblasts
Staining:High
Intensity: Strong
Mucosal lymphoid cells
Staining:High
Intensity: Strong
Quantity:>75%
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_A_7_3.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170004-CHD3
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CHD3
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-6G3
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Snijders Blok-Campeau Syndrome; Stuttering
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- ZFH; Mi-2a; SNIBCPS; Mi2-ALPHA
- Gene ID
- 1107
- UniProt ID
- Q12873
- Long Name
- Chromodomain helicase DNA binding protein 3
- Cellular Localization
- Nucleus, PML body, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
- Post Translation Modifications
- Sumoylation at Lys-1971 results in dissociation from chromatin and suppression of transcriptional repression. (Q12873-CHD3_HUMAN)
Modification sites at PhosphoSitePlus (Q12873)
Modification sites at neXtProt (NX_Q12873)
Glycosylation from GlyGen (Q12873) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_005256484.1; XP_005256485.1
- Function
- ATP-dependent helicase that binds and distorts nucleosomal DNA. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin.
Involved in transcriptional repression as part of the NuRD complex.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CHD3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-299 | Human Anti-CHD3 Recombinant Antibody (clone D404) | ELISA, IP-MS | Human IgG |
| MOB-0486CT | Recombinant Mouse anti-Human CHD3 Monoclonal antibody (EML044) | IHC-P, WB | |
| ZG-0416F | Mouse Anti-CHD3 Recombinant Antibody (ZG-0416F) | WB, ICC | Mouse IgG |
| VS3-QX232 | Mouse Anti-CHD3 Recombinant Antibody (clone 4B1-F1-G4) | WB, IHC, FC | Mouse IgG1 |
| VS3-QX233 | Mouse Anti-CHD3 Recombinant Antibody (clone 7F6-G2-E5) | WB, ICC, IP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0685 | Hi-Affi™ Rabbit Anti-CHD3 Recombinant Antibody (clone DS685AB) | WB, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY443 | Anti-CHD3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1425 | Anti-Mouse CHD3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1424 | Anti-Human CHD3 Immunohistochemistry Kit | IHC |
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