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    Rabbit Anti-CHD3 Recombinant Antibody (clone R05-6G3) (CAT#: VS3-XY3313)

    This product is a recombinant rabbit antibody targeting the CHD3 (Chromodomain helicase DNA binding protein 3), which involves in transcriptional repression as part of the NuRD complex. It recognizes and binds to human CDH3. It also has cross-reactivity against mouse and rat CHD3. It has been determined using Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence.

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    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
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    Purity :
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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1674_H9_32_selected.jpg
    Figure 2 IHC staining of human cerebral cortex
    Figure 2 IHC staining of human cerebral cortex
    (Immunohistochemical staining of human cerebral cortex shows distinct nuclear positivity in neuronal cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/ihc_selected.jpg
    Figure 3 IF staining of human cell line REH
    Figure 3 IF staining of human cell line REH
    (Immunofluorescent staining of human cell line REH shows localization to nucleoplasm & nucleoli.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1676_H9_2_red_green.jpg
    Figure 4 IF staining of human cell line SiHa
    Figure 4 IF staining of human cell line SiHa
    (Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & nucleoli.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1712_C4_3_cr5804e5a4d2793_red_green.jpg
    Normal Tissue
    Figure 5 Cerebral cortex
    Figure 5 Cerebral cortex
    (Neuronal cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_B_8_5.jpg
    Figure 6 Colon
    Figure 6 Colon
    (Endocrine cells
    Staining:Medium
    Intensity: Moderate
    Endothelial cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Enterocytes
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Fibroblasts
    Staining:High
    Intensity: Strong
    Mucosal lymphoid cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Peripheral nerve/ganglion
    Staining:High
    Intensity: Strong
    Quantity:>75%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_A_7_3.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Leydig cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Sertoli cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_A_6_6.jpg
    RNA Expression
    Figure 8 RNA cell line category: Low cell line specificity
    Figure 8 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170004-CHD3

    Specifications

    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human, Mouse and Rat CHD3
    • Species Reactivity
    • Human, Mouse, Rat
    • Clone
    • R05-6G3
    • Applications
    • WB, IHC-P, ICC, IF, FC
    • Related Disease
    • Snijders Blok-Campeau Syndrome; Stuttering

    Product Property

    • Clonality
    • Monoclonal
    • Aggregation
    • < 5%
    • Format
    • Liquid
    • Buffer
    • PBS (pH 7.4), 150 mM NaCl and 50% glycerol
    • Preservative
    • 0.02% sodium azide
    • Storage
    • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • WB: 1/500-1/1000
      IHC: 1/50-1/100
      IF: 1/50-1/200
      FC: 1/50-1/100

    Target

    • Alternative Names
    • ZFH; Mi-2a; SNIBCPS; Mi2-ALPHA
    • Long Name
    • Chromodomain helicase DNA binding protein 3
    • Cellular Localization
    • Nucleus, PML body, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
    • Post Translation Modifications
    • Sumoylation at Lys-1971 results in dissociation from chromatin and suppression of transcriptional repression. (Q12873-CHD3_HUMAN)
      Modification sites at PhosphoSitePlus (Q12873)
      Modification sites at neXtProt (NX_Q12873)
      Glycosylation from GlyGen (Q12873) 1 site, 1 O-linked glycan (1 site)
    • Function
    • ATP-dependent helicase that binds and distorts nucleosomal DNA. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin.
      Involved in transcriptional repression as part of the NuRD complex.

    Recommended Products

    Isotype Control

    Recommended Dilution Buffer

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "CHD3"

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    CAT Product Name Application Type
    DrMAB-299 Human Anti-CHD3 Recombinant Antibody (clone D404) ELISA, IP-MS Human IgG
    ZG-0416F Mouse Anti-CHD3 Recombinant Antibody (ZG-0416F) WB, ICC Mouse IgG
    VS3-QX232 Mouse Anti-CHD3 Recombinant Antibody (clone 4B1-F1-G4) WB, IHC, FC Mouse IgG1
    VS3-QX233 Mouse Anti-CHD3 Recombinant Antibody (clone 7F6-G2-E5) WB, ICC, IP Mouse IgG1
    VS3-CJ76 Mouse Anti-CHD3 Recombinant Antibody (VS3-CJ76) WB, ELISA Mouse IgG

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