Mouse Anti-CHRNA4 Recombinant Antibody (CH460)
CAT#: VS-0326-YC460
The anti-CHRNA4 recombinant antibody (CH460) reacts with human CHRNA4. CHRNA4 is a major subunit of the high-affinity (α4)2(β2)3 nicotinic receptor in the central nervous system. It mediates fast excitatory synaptic transmission and is the primary target for nicotine addiction. Mutations in CHRNA4 are associated with Autosomal Dominant Nocturnal Frontal Lobe Epilepsy (ADNFLE), due to altered receptor desensitization and excitability. The anti-CHRNA4 antibody (CH460) has been tested in Enzyme-linked Immunosorbent Assay (ELISA) and Flow Cytometry (FC).
Gene Expression
Normal Tissue
Figure 1 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34064/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34064/70741_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34064/70741_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34064/70741_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34064/70741_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34064/70741_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34064/70741_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enriched (U-2 OS)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101204-CHRNA4
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CHRNA4
- Species Reactivity
- Human
- Clone
- CH460
- Applications
- ELISA, FC
- Conjugate
- The unconjugated, Biotin and FITC formats are available.
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- ELISA (1/5000-1/20000), FC (1/200-1/400)
Target
- Alternative Names
- Cholinergic Receptor Nicotinic Alpha 4 Subunit; Cholinergic Receptor, Nicotinic, Alpha Polypeptide 4; Cholinergic Receptor, Nicotinic, Alpha 4 (Neuronal); Cholinergic Receptor, Nicotinic Alpha 4; NACRA4; Neuronal Nicotinic Acetylcholine Receptor Alpha-4 Subunit; Acetylcholine Receptor, Nicotinic, Alpha 4 (Neuronal);
- Gene ID
- 1137
- UniProt ID
- P43681
- Long Name
- Cholinergic Receptor Nicotinic Alpha 4 Subunit
- Post Translation Modifications
- Unmodified
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CHRNA4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3787z | Mouse Anti-CHRNA4 Recombinant Antibody (clone 19G3) | WB, ELISA | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0698 | Hi-Affi™ Rabbit Anti-CHRNA4 Recombinant Antibody (clone DS698AB) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY2047 | Rabbit Anti-CHRNA4 Recombinant Antibody (clone R03-1A5) | WB, IP | Rabbit IgG |
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