Human Anti-CLDN18 Recombinant Antibody (clone 25E3) (CAT#: ZG-0140U)
Human anti-CLDN18 antibody (clone 25E3) displayed high specificity against the CLDN18 protein of Human.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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FC
Figure 1 Human Anti-CLDN18 Antibody (ZG-0140U) in FC
Untransfected KATO3 cells (green line), transfected Human CLDN18.2 KATO3 stable cells (red line) and transfected Human CLDN18.1 NIH-3T3 stable cells (blue line) were stained with anti-CLDN18 antibody (2µg/1*106 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody (ZG-0140U) and analyzed with flow cytometry.
ELISA
Figure 2 Human Anti-CLDN18 Antibody (ZG-0140U) in ELISA
The Binding Activity of Human CLDN18.2 with Anti-CLDN18 recombinant antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLDN18.2 (ZG-0140U) at 5 μg/mL can bind Anti-CLDN18 recombinant antibody, the EC50 is 2.947-8.782 ng/mL.
Specifications
- Immunogen
- Recombinant Human CLDN18 protein
- Host Species
- Human
- Type
- Humanized IgG1
- Specificity
- Human CLDN18
- Species Reactivity
- Human
- Clone
- 25E3
- Applications
- ELISA, FC
- Conjugate
- Unconjugated
- Related Disease
- Bile Duct Cancer and Urachus Cancer
Product Property
- Purification
- Affinity Purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Flow Cytometry. Specific conditions should be optimized by the end user.
FC:1:50-1:200
Target
- Alternative Names
- SFTA5; SFTPJ; claudin-18; surfactant associated 5; surfactant associated protein J; surfactant, pulmonary associated protein J
- Gene ID
- 51208
- UniProt ID
- P56856
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-215-F(E) | Recombinant Anti-human CLDN18 Antibody Fab Fragment | IP, IF, FuncS | Fab |
| MHH-215-F(E) | Recombinant Human Anti-human CLDN18 Antibody Fab Fragment | WB, IF, FuncS | Fab |
Customer Reviews and Q&As
Customer Review
Q&As
Knockout Validation Aid
This antibody perfectly suited our needs for validating knockdown experiments. Its reliability was confirmed by the clear specificity and lack of signal in knockout samples.
Ideal for Routine Lab Use
Incorporating this antibody into our routine lab procedures has been incredibly beneficial. Consistency, reliability, and clear results have streamlined our workflows significantly.
Cost-Efficient
Considering its performance and versatility across multiple applications, this antibody provides excellent value. It's a smart investment for any lab looking for high-quality reagents.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# ZG-0140U, RRID: AB_3112064)
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For Research Use Only. Not For Clinical Use.
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