Mouse Anti-Clock Recombinant Antibody (ZG-0432F)
CAT#: ZG-0432F
This product is a mouse antibody that recognizes Clock.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-Clock Recombinant Antibody (ZG-0432F) in WB.
Western blot analysis was performed using clock monoclonal antibody against HEK293 cell lysates transfected with CLOCK hIgGFc.
IF
Figure 2 Mouse Anti-Clock Recombinant Antibody (ZG-0432F) in IF.
Immunofluorescence analysis of Hela cells using clock monoclonal antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1867/93_G2_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27565/218_G11_1_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27565/58303_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1867/7540_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27565/58303_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1867/7540_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1867/7540_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134852-CLOCK
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Specifications
- Immunogen
- Recombinant fragment of human Clock expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human Clock
- Species Reactivity
- Human
- Applications
- Western Blot, Immunofluorescence, Enzyme-linked Immunosorbent Assay
- Conjugate
- NONE
Product Property
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunofluorescence: 1/200-1/1000.
ELISA: 1/10000
Target
- Alternative Names
- Clock Circadian Regulator; Class E Basic Helix-Loop-Helix Protein 8; EC 2.3.1.48; BHLHe8; Circadian Locomoter Output Cycles Kaput Protein; Circadian Locomoter Output Cycles Protein Kaput; Clock (Mouse) Homolog; CLOCK; BHLHE8; KIAA0334; Circadian locomoter output cycles protein kaput; hCLOCK; Class E basic helix-loop-helix protein 8; bHLHe8
- Gene ID
- 9575
- UniProt ID
- O15516
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "CLOCK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4050z | Mouse Anti-CLOCK Recombinant Antibody (clone 31B8) | WB, IP, IF, ELISA | Mouse IgG2b |
| MOB-0949CT | Recombinant Mouse anti-Human CLOCK Monoclonal antibody (759D2b) | Dot, WB | |
| MRO-0371-CN | Rabbit Anti-CLOCK Recombinant Antibody (clone JA12-33) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-QX250 | Mouse Anti-CLOCK Recombinant Antibody (clone 8F7) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS7-HM752 | Mouse Anti-CLOCK Recombinant Antibody (clone CBL168HM) | WB, IF, ICC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0730 | Hi-Affi™ Rabbit Anti-CLOCK Recombinant Antibody (clone DS730AB) | WB, IHC, ICC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY474 | Anti-CLOCK Immunohistochemistry Kit | IHC | |
| VS-0525-XY1520 | Anti-Mouse CLOCK Immunohistochemistry Kit | IHC | |
| VS-0525-XY1519 | Anti-Human CLOCK Immunohistochemistry Kit | IHC |
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