Mouse Anti-COL1A1 Recombinant Antibody (VS4-WK117)
CAT#: VS4-WK117
This product is a mouse monoclonal antibody that recognizes COL1A1 and can be used in ELISA, IHC.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12111/1934_F8_3_cr5d1ca7aa8d4b0_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows extracellular positivity.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12111/1936_A7_4_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/32976_B_7_5.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/32976_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells
Staining:High
Intensity: Strong
Quantity: 75%-25% Preleptotene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/32976_A_5_6.jpg
Normal Tissue
Figure 7 Skin
Fibroblasts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Keratinocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/32976_B_9_1.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (ASC TERT1, BJ, fHDF/TERT166, HSkMC, U-138 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000108821-COL1A1
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Specifications
- Immunogen
- Synthetic Peptide of Collagen I
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG
- Specificity
- Human COL1A1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- ELISA, IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- The antibody was purified from mouse ascites by immunogen affinity chromatography.
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS containing 50% glycerol, pH 7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at -20°C or -80°C. Avoid repeated freeze.
- Shipping
- Ice packs
Applications
- Application Notes
- Recommended dilution: IHC:1:50-1:200
Target
- Alternative Names
- Collagen Type I Alpha 1 Chain; Collagen, Type I, Alpha 1; Alpha-1 Type I Collagen; Collagen Of Skin, Tendon And Bone, Alpha-1 Chain; Collagen Alpha-1(I) Chain Preproprotein; Type I Procollagen Alpha 1 Chain; Collagen Alpha 1 Chain Type I; Pro-Alpha-1 Collagen Type 1; Collagen Alpha-1(I) Chain;
- Gene ID
- 1277
- UniProt ID
- P02452
- Long Name
- Collagen Type I Alpha 1 Chain
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "COL1A1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-706-VHH | Recombinant Anti-Human COL1A1 VHH Single Domain Antibody | IHC, IP, FC, Neut, FUNC | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0754 | Hi-Affi™ Rabbit Anti-COL1A1 Recombinant Antibody (clone DS754AB) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0374-CN | Rabbit Anti-COL1A1 Recombinant Antibody (clone CBACN-151) | WB, IHC | Rabbit IgG |
| ZG-0623F | Rabbit Anti-COL1A1 Recombinant Antibody (ZG-0623F) | ELISA, RIA, WB, IHC-P, IF, RIA | Rabbit IgG |
| ZG-0625F | Rabbit Anti-COL1A1 Recombinant Antibody (ZG-0625F) | IHC-P, IF, ELISA, RIA, WB | Rabbit IgG |
| ZG-0626F | Rabbit Anti-COL1A1 Recombinant Antibody (ZG-0626F) | IHC-P, IF, ELISA, RIA, WB | Rabbit IgG |
| VS3-CJ278 | Rabbit Anti-COL1A1 Recombinant Antibody (VS3-CJ278) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY500 | Anti-COL1A1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1581 | Anti-Human COL1A1 Immunohistochemistry Kit | IHC |
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