Rabbit Anti-CRK Recombinant Antibody (clone R83-7X-4)
CAT#: VS3-FY2131
This is a rabbit recombinant monoclonal antibody that is involved in the recognition of human CRK. It cross-reacts with mouse, rat. We recommend antibody dilution: western blot (dilution 1/500-1/1000), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/50), flow cytometry (dilution 1/50).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68087/1304_C12_5_selected.jpg
Normal Tissue
Figure 2 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10485/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10485/32478_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10485/32478_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10485/32478_A_9_5.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000167193-CRK
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CRK
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R83-7X-4
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation, Flow Cytometry
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 34kDa, while the observed molecular weight stands at 38kDa.
- Related Disease
- Sarcoma and Chromosome 17P13.3, Centromeric, Duplication Syndrome
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/50
Flow Cytometry: 1/50
Target
- Alternative Names
- CRK Proto-Oncogene, Adaptor Protein; V-Crk Avian Sarcoma Virus CT10 Oncogene Homolog; Proto-Oncogene C-Crk; P38; V-Crk Sarcoma Virus CT10 Oncogene-Like Protein; Adapter Molecule Crk; CRKII
- Gene ID
- 1398
- UniProt ID
- P46108
- Sequence Similarities
- Belongs to the CRK family.
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane
- Post Translation Modifications
- Phosphorylated on Tyr-221 upon cell adhesion.
Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain.
This leads finally to the down-regulation of the Crk signaling pathway (PubMed:17515907).
Isoform Crk-II: Phosphorylated by KIT ().
- Protein Refseq
- NP_005197.3; NP_058431.2
- Function
- Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1.
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Related Signaling Pathways
ErbB Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CRK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0807 | Hi-Affi™ Rabbit Anti-CRK Recombinant Antibody (clone DS807AB) | ICC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0438F | Mouse Anti-CRK Recombinant Antibody (ZG-0438F) | WB | Mouse IgG |
| ZG-0439F | Mouse Anti-CRK Recombinant Antibody (ZG-0439F) | WB | Mouse IgG |
| VS7-HM769 | Mouse Anti-CRK Recombinant Antibody (clone CBL172HM) | WB, IHC-p, IF, ICC, FCM, ELISA | Mouse IgG |
| VS7-HM770 | Mouse Anti-CRK Recombinant Antibody (clone CBL173HM) | WB, IHC-p, IF, ICC, FCM, ELISA | Mouse IgG |
| VS7-HM771 | Mouse Anti-CRK Recombinant Antibody (clone CBL174HM) | WB | Mouse IgG |
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