Rabbit Anti-CRK Recombinant Antibody (clone R83-7X-4) (CAT#: VS3-FY2131)
This is a rabbit recombinant monoclonal antibody that is involved in the recognition of human CRK. It cross-reacts with mouse, rat. We recommend antibody dilution: western blot (dilution 1/500-1/1000), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/50), flow cytometry (dilution 1/50).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68087/1304_C12_5_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68087/1304_C12_5_selected.jpg
Figure 2 IHC staining of human small intestine
(Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10485/ihc_selected.jpg
(Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10485/ihc_selected.jpg
Figure 3 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10485/32478_A_7_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10485/32478_A_7_3.jpg
Figure 4 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10485/32478_A_8_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10485/32478_A_8_4.jpg
Figure 5 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10485/32478_A_9_5.jpg
(Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10485/32478_A_9_5.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000167193-CRK
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000167193-CRK
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CRK
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R83-7X-4
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation, Flow Cytometry
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 34kDa, while the observed molecular weight stands at 38kDa.
- Related Disease
- Sarcoma and Chromosome 17P13.3, Centromeric, Duplication Syndrome
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/50
Flow Cytometry: 1/50
Target
- Alternative Names
- CRK Proto-Oncogene, Adaptor Protein; V-Crk Avian Sarcoma Virus CT10 Oncogene Homolog; Proto-Oncogene C-Crk; P38; V-Crk Sarcoma Virus CT10 Oncogene-Like Protein; Adapter Molecule Crk; CRKII
- Gene ID
- 1398
- UniProt ID
- P46108
- Sequence Similarities
- Belongs to the CRK family.
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane
- Post Translation Modifications
- Phosphorylated on Tyr-221 upon cell adhesion.
Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain.
This leads finally to the down-regulation of the Crk signaling pathway (PubMed:17515907).
Isoform Crk-II: Phosphorylated by KIT ().
- Protein Refseq
- NP_005197.3; NP_058431.2
- Function
- Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1.
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
ErbB Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CRK"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0407CT | Recombinant Mouse anti-Human CRK Monoclonal antibody (3320D2b) | Dot, WB | |
| ZG-0438F | Mouse Anti-CRK Recombinant Antibody (ZG-0438F) | WB | Mouse IgG |
| VS3-XY526 | Mouse Anti-CRK Recombinant Antibody (clone 3G11C1) | ELISA, WB, IHC, FC, ICC | Mouse IgG2b |
| VS3-XY527 | Mouse Anti-CRK Recombinant Antibody (clone 3G11E8) | ELISA, WB, IHC, FC, ICC | Mouse IgG2b |
| VS4-WK124 | Mouse Anti-CRK Recombinant Antibody (VS4-WK124) | ELISA, WB | Mouse IgG2b |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0807 | Hi-Affi™ Rabbit Anti-CRK Recombinant Antibody (clone DS807AB) | ICC, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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