Recombinant Mouse Anti-CyRPA Antibody (8A7) (CAT#: PABC-476)
This product is a recombinant Mouse antibody that can recognize CyRPA. 8A7 directly prevents RH5 binding to CyRPA. Antibody 8A7 bound to CyRPA, disrupting the Rh5/CyRPA complex, and CyRPA observed in the earlier fractions is in complex with the antibody. The antibody 8A7 epitope lies outside the interface of the tetrameric CyRPA in the crystallographic asymmetric unit.
Specific Inquiry
IB
Figure 1 Immunoblot of inhibitory (8A7) and non-inhibitory (7A6) monoclonal antibodies against proteins from CyRPA-HA tagged transgenic P. falciparum schizonts in reduced (R) and non-reduced (NR) condition.
The P. falciparum parasites expressed haemagglutinin-tagged CyRPA protein.
Chen, L., Xu, Y., Wong, W., Thompson, J. K., Healer, J., Goddard-Borger, E. D., ... & Cowman, A. F. (2017). Structural basis for inhibition of erythrocyte invasion by antibodies to Plasmodium falciparum protein CyRPA. Elife, 6, e21347.
Inhib
Figure 2 Anti-CyRPA monoclonal antibodies inhibit P. falciparum growth and interaction of PfRh5.
In vitro growth inhibition (GIA) assays were performed to assess the abilities of the monoclonal antibodies raised against recombinant CyRPA to block P. falciparum parasite growth in human erythrocytes. Assays were performed twice in triplicate and error bars denote SD of the mean of 6 values. Anti-Rh5 mAb 4G4 (not inhibitory) and antiRipr mAb 5G6 (inhibitory) are included as GIA controls.
Chen, L., Xu, Y., Wong, W., Thompson, J. K., Healer, J., Goddard-Borger, E. D., ... & Cowman, A. F. (2017). Structural basis for inhibition of erythrocyte invasion by antibodies to Plasmodium falciparum protein CyRPA. Elife, 6, e21347.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Species Reactivity
- P. falciparum
- Clone
- 8A7
- Applications
- IB, Inhib, ELISA, WB
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunoblotting, Inhibition and Western Blot. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
- Alternative Names
- Plasmodium falciparum protein CyRPA; CyRPA
- UniProt ID
- Q8IFM8
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone 8A7"
See other products for "CyRPA"
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-476 | Recombinant Mouse Anti-CyRPA Antibody Fab Fragment (8A7) | ELISA, WB, Inhib | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-476 | Recombinant Mouse Anti-CyRPA Antibody scFv Fragment (8A7) | ELISA, WB, Inhib | Mouse scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0923-XY5 | Recombinant Chicken Anti-CyRPA Antibody (clone Cy.004) | ELISA, SPR, Inhib | Chicken IgY |
| VS-0923-XY6 | Recombinant Mouse Anti-CyRPA Antibody (clone Cy.002) | ELISA, SPR | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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