Recombinant Mouse Anti-Cyt c Antibody (2-96-12 ) (CAT#: FAMAB-0062CQ)
This product is a recombinant mouse antibody clone 2-96-12, which specifically binds to Cytochrome c.
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ELISA
Figure 1 Titration of monoclonal antibodies by ELISA.
Wells in plates were coated with yeast ferri-iso-l-cytochrome c and incubated with serially diluted hybridoma supernatants for 16-18 h at 5 centigrade. The details of the procedure are described in the Miniprint. Hybridomas supernatants are: ●, 4-74-6; ▼, 10-28-86; ■, 2-34-19; ∆, 2-96-12; ▲, 4-145-10; and □, 39-14. M-1 medium was used to dilute hybridoma supernatants with the exception that M-2 medium was used for mAb 10-28-86. Ten-fold (10 X) concentrated monoclonals were prepared from partially purified monoclonals (see the Miniprint). The reaction time of horseradish peroxidase was 2 h with the exception that it was 1 h for mAb 10-28-86. Ordinate, absorbance at 492 nm.
Silvestri, I., & Taniuchi, H. (1988). A study of fine specificity of monoclonal antibodies to yeast iso-1-cytochrome c. Journal of Biologic
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Cytochrome c
- Species Reactivity
- Yeast
- Clone
- 2-96-12
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- cyt c; Cytochrome c
Related Resources
p53 Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0063CQ | Recombinant Mouse Anti-Cyt c Antibody (4-74-6) | ELISA | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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