Rabbit Anti-DAB2 Recombinant Antibody (clone R04-2A1)
CAT#: VS3-FY1994
The R04-2A1 recombinant monoclonal antibody is involved in the recognition of human DAB2. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, ICC/IF, IP. This DAB2 antibody is guaranteed for western blot analysis of DAB2 in Hela, A549, HL-60, U2OS, C6 lysates and immunocytochemistry analysis of DAB2 (green) in hela using DAB2 antibody.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to nucleoli fibrillar center, plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28888/785_H11_7_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9314/996_D1_1_selected.jpg
Normal Tissue
Figure 3 Placenta
Decidual cells Staining: Low Intensity: Weak Quantity:>75% Hofbauer cells Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - cell body Staining: Medium Intensity: Moderate Quantity:>75% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28888/134186_A_1_7.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, fHDF/TERT166, TIME, U-138 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000153071-DAB2
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WB
Figure 1 Anti-DAB2 Antibody (VS3-FY1994) in WB.
The DAB2 expression in Hela, A549, HL-60, U2OS, C6 lysates were detected using the anti-DAB2 by western blot assay.
ICC
Figure 2 Anti-DAB2 Antibody (VS3-FY1994) in ICC.
Immunocytochemistry staining of DAB2 (green) in hela using the anti-DAB2 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
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Specifications
- Immunogen
- Synthetic peptide of human DAB2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat DAB2
- Species Reactivity
- Human, Rat
- Clone
- R04-2A1
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 82 kDa, while the observed molecular weight stands at 96 kDa.
- Related Disease
- Teratocarcinoma and Hypercholesterolemia, Familial, 4
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- DAB2, Clathrin Adaptor Protein; Differentially Expressed In Ovarian Carcinoma 2; Differentially-Expressed Protein 2; Adaptor Molecule Disabled-2; DOC-2; DOC2; Disabled (Drosophila) Homolog 2 (Mitogen-Responsive Phosphoprotein)
- Gene ID
- 1601
- UniProt ID
- P98082
- Cellular Localization
- Coated pit, Cytoplasm, Cytoplasmic vesicle, Membrane
- Post Translation Modifications
- Phosphorylated.
Phosphorylation during mitosis is leading to membrane displacement ().
(P98082-DAB2_HUMAN)
Modification sites at PhosphoSitePlus (P98082)
Modification sites at neXtProt (NX_P98082)
Glycosylation from GlyGen (P98082) 13 sites, 1 O-linked glycan (13 sites)
- Protein Refseq
- NP_001231800.1; NP_001334.2
- Function
- Adapter protein that functions as a clathrin-associated sorting protein (CLASP) required for clathrin-mediated endocytosis of selected cargo proteins. Can bind and assemble clathrin, and binds simultaneously to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and cargos containing non-phosphorylated NPXY internalization motifs, such as the LDL receptor, to recruit them to clathrin-coated pits. Can function in clathrin-mediated endocytosis independently of the AP-2 complex.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "DAB2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0918 | Hi-Affi™ Rabbit Anti-DAB2 Recombinant Antibody (clone DS918AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK178 | Mouse Anti-DAB2 Recombinant Antibody (clone H7-C4) | WB, ELISA | Mouse IgG1 |
| VS13-YC285 | Rabbit Anti-DAB2 Recombinant Antibody (VS13-YC285) | WB, IHC-P, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1891 | Anti-DAB2 Immunohistochemistry Kit | IHC |
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