Rabbit Anti-DAPK1 Recombinant Antibody (clone R01-5J9) (CAT#: VS3-XY3330)
This is a recombinant rabbit antibody targeting the DAPK1 (Death associated protein kinase 1). It recognizes and binds to human DAPK1. It also has cross-reactivity against mouse and rat DAPK1. It has been detected by Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin and Immunofluorescence is 1/500-1/1000, 1/50-1/100 and 1/50-1/200, respectively.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37302/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37302/if_selected.jpg
Figure 2 IHC staining of human vulva/anal skin
(Immunohistochemical staining of human vulva/anal skin shows distinct cytoplasmic positivity in basal layer of epidermis.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40472/125349_B_5_8_selected.jpg
(Immunohistochemical staining of human vulva/anal skin shows distinct cytoplasmic positivity in basal layer of epidermis.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40472/125349_B_5_8_selected.jpg
Figure 3 IHC staining of human heart muscle
(Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48436/ihc_selected.jpg
(Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48436/ihc_selected.jpg
Figure 4 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37302/ihc_selected.jpg
(Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37302/ihc_selected.jpg
Figure 5 Skin
(Epidermal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40472/125349_B_5_8.jpg
(Epidermal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40472/125349_B_5_8.jpg
Figure 6 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40472/125349_A_7_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40472/125349_A_7_4.jpg
Figure 7 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40472/125349_A_8_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40472/125349_A_8_5.jpg
Figure 8 RNA cell line category: Cell line enhanced (HMC-1, HUVEC TERT2)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196730-DAPK1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196730-DAPK1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat DAPK1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-5J9
- Applications
- WB, IHC-P, ICC, IF
- Related Disease
- Pancreatic Ductal Adenocarcinoma; Cervical Squamous Cell Carcinoma
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- DAPK; ROCO3
- Gene ID
- 1612
- UniProt ID
- P53355
- Long Name
- Death associated protein kinase 1
- Cellular Localization
- Cytoplasm, cytoskeleton
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (P53355)
Modification sites at neXtProt
- Protein Refseq
- XP_005251814.1; XP_047278844.1
- Function
- Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting.
The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles.
Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function.
Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Related Resources
Bladder Cancer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1513MZ | Recombinant Mouse Anti-Human DAPK1 Antibody (clone ELQT409) | ELISA, IP | Mouse antibody |
| VS3-QX315 | Mouse Anti-DAPK1 Recombinant Antibody (clone 12B2) | WB, IHC | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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