Rabbit Anti-DAPK1 Recombinant Antibody (clone R01-5J9)
CAT#: VS3-XY3330
This is a recombinant rabbit antibody targeting the DAPK1 (Death associated protein kinase 1). It recognizes and binds to human DAPK1. It also has cross-reactivity against mouse and rat DAPK1. It has been detected by Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin and Immunofluorescence is 1/500-1/1000, 1/50-1/100 and 1/50-1/200, respectively.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37302/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human vulva/anal skin
Immunohistochemical staining of human vulva/anal skin shows distinct cytoplasmic positivity in basal layer of epidermis.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40472/125349_B_5_8_selected.jpg
Normal Tissue
Figure 3 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48436/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37302/ihc_selected.jpg
Normal Tissue
Figure 5 Skin
Epidermal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40472/125349_B_5_8.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40472/125349_A_7_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40472/125349_A_8_5.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (HMC-1, HUVEC TERT2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196730-DAPK1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat DAPK1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-5J9
- Applications
- WB, IHC-P, ICC, IF
- Related Disease
- Pancreatic Ductal Adenocarcinoma; Cervical Squamous Cell Carcinoma
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- DAPK; ROCO3
- Gene ID
- 1612
- UniProt ID
- P53355
- Long Name
- Death associated protein kinase 1
- Cellular Localization
- Cytoplasm, cytoskeleton
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (P53355)
Modification sites at neXtProt
- Protein Refseq
- XP_005251814.1; XP_047278844.1
- Function
- Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting.
The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles.
Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function.
Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells.
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Related Diseases
Bladder Cancer
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "DAPK1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1513MZ | Recombinant Mouse Anti-Human DAPK1 Antibody (clone ELQT409) | ELISA, IP | Mouse antibody |
| VS3-QX315 | Mouse Anti-DAPK1 Recombinant Antibody (clone 12B2) | WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0923 | Hi-Affi™ Rabbit Anti-DAPK1 Recombinant Antibody (clone DS923AB) | WB, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY617 | Anti-DAPK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1898 | Anti-Mouse DAPK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1897 | Anti-Human DAPK1 Immunohistochemistry Kit | IHC |
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