Anti-DDX5 Antibody Prodrug, Protease Activated (1G3)
CAT#: VS-1025-YC120
The Anti-DDX5 Antibody Prodrug, Protease Activated (1G3) is an antibody prodrug comprised of a recombinant human antibody targeting human DDX5, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to DDX5 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to DDX5 effectively. DDX5 (DEAD-box Helicase 5) is an RNA helicase that is often overexpressed in human tumors and acts as a co-regulator of transcription factors, including those in the Wnt and steroid receptor pathways. Its heightened activity can promote cell proliferation and survival, and it contributes to the malignancy of cancers like colorectal and prostate cancer.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20043/278_G2_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71154/1949_B4_1_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5868/642_D2_1_selected.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20043/45622_A_4_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20043/45622_A_8_8.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20043/45892_B_8_5.jpg
Normal Tissue
Figure 7 Colon
Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Fibroblasts Staining: High Intensity: Strong Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20043/45622_A_9_3.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20043/45622_A_8_5.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108654-DDX5
❮
❯
Specifications
- Host Animal
- Human
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human DDX5
- Target
- DDX5
- Species Reactivity
- Human
- Isotype
- IgG
- Clone
- 1G3
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified.
- Alternative Names
- DEAD (Asp-Glu-Ala-Asp) Box Helicase 5; G17P1; HLR1; ATP-Dependent RNA Helicase DDX5; EC 3.6.4.13; DEAD Box-5; EC 3.6.1; HUMP68; HELR; P68
- Gene ID
- 1655
- UniProt ID
- P17844
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-1025-YC120. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 1G3"
- CAT
- Product Name
See other products for "DDX5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2288CT | Recombinant Mouse anti-Human DDX5 Monoclonal antibody (70) | WB, Dot |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0962 | Hi-Affi™ Rabbit Anti-DDX5 Recombinant Antibody (clone DS962AB) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1932 | PE-A*02:01/Human DDX5 (YLLPAIVHI) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2359 | APC-A*02:01/Human DDX5 (YLLPAIVHI) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0197-CN | Human Anti-DDX5 Recombinant Antibody (clone 1G3) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0197-CN-S(P) | Human Anti-DDX5 Recombinant Antibody (clone 1G3); scFv Fragment | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0197-CN-F(E) | Human Anti-DDX5 Recombinant Antibody (clone 1G3); Fab Fragment | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0467-CN | Rabbit Anti-DDX5 Recombinant Antibody (clone JM52-30) | WB, IF, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0634J | Rabbit Anti-DDX5 Recombinant Antibody (clone 2C3) | ELISA, WB, IHC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY449 | Recombinant Rabbit Anti-DDX5 Antibody (clone R04-2K2) | WB, IHC-F, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC771 | AbPlus™ Anti-DDX5 Magnetic Beads (VS-0724-YC771) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY648 | Anti-DDX5 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1962 | Anti-Human DDX5 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: WB, IHC, FC, Cyt, ELISA
Application:
Application: ELISA, FC, WB, FuncS
Application: ELISA, FC, Inhib
-4.jpg)
Application: FC, FuncS, IA, IF, IP, IHC
-2.png)
Application: ELISA
Application: ELISA, Block, WB, FC, IP
Application: IF, ICC, WB, IHC-P, IP
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
