Mouse Anti-DNMT3L Recombinant Antibody (clone B0-5)
CAT#: MRO-0489-CN
This antibody is a mouse monoclonal antibody that is capable of binding to DNMT3L.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55234/1700_E7_31_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66780/148791_B_9_5.jpg
Normal Tissue
Figure 3 Pancreas
Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Pancreatic endocrine cells Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66780/148791_A_2_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66780/148791_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66780/148791_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enriched (BEWO)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000142182-DNMT3L
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Clone
- B0-5
- Applications
- WB, IF, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Protein G affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS, pH 7.4, 1% BSA, 40% Glycerol
- Preservative
- 0.05% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Specific DNMT3L Detection
We utilized the B0-5 clone for detecting DNMT3L in mouse tissues. This Recombinant Mouse Antibody worked perfectly in Western Blot and Immunohistochemistry. It specifically identifies the DNMT3L protein, allowing us to map its expression during development. The Protein G purification ensures a clean product.
Great for Immunofluorescence
This antibody provided excellent results in Immunofluorescence assays. The staining of nuclear DNMT3L was distinct and specific. We appreciate that it is a recombinant antibody, which guarantees batch-to-batch consistency. It arrived in a buffer containing glycerol, making it ready for -20°C storage immediately.
Q&As
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What is the target specificity of this antibody?
A: This Recombinant Mouse Antibody (clone B0-5) is capable of binding to DNMT3L. It specifically recognizes the protein and has been validated for detecting it in Western Blot, Immunofluorescence (IF), and Immunohistochemistry (IHC) applications.
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How is the antibody supplied and stored?
A: The antibody is supplied in TBS, pH 7.4, with 1% BSA, 40% Glycerol, and 0.05% Sodium azide. This formulation allows for storage at -20°C without freezing solid, preventing damage from freeze/thaw cycles. It is purified with Protein G affinity chromatography.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "DNMT3L"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0565CT | Recombinant Mouse anti-Human DNMT3L Monoclonal antibody (T228-0) | ICC, IF, IHC-Fr, IHC-P, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-A1927-YJ | Mouse Anti-DNMT3L Recombinant Antibody (clone CBS389) | WB, IHC, ICC, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0422-LC60 | Human Anti-DNMT3L Recombinant Antibody (clone hN117/9.5) | WB, IF, IHC | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0422-LC61 | Rabbit Anti-DNMT3L Recombinant Antibody (clone rN117/9.5) | WB, IF, IHC | Rabbit IgG |
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