Rabbit Anti-DUSP1 Recombinant Antibody (clone R03-5D1)
CAT#: VS3-XY3339
It is a recombinant rabbit antibody that recognizes human DUSP1 (Dual specificity phosphatase 1). It is capable of recognizing human, mouse and rat DUSP1. It has been used in a variety of applications, such as Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoli & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69577/1586_C7_2_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced (HHSteC, hTCEpi, REH)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120129-DUSP1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat DUSP1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R03-5D1
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Related Disease
- Intellectual Developmental Disorder, X-Linked 108; Ovarian Cancer
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- HVH1; MKP1; CL100; MKP-1; PTPN10
- Gene ID
- 1843
- UniProt ID
- P28562
- Long Name
- Dual specificity phosphatase 1
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylation at Ser-359 and Ser-364 by MAPK1/ERK2 and MAPK3/ERK1 reduces its rate of degradation. (P28562-DUS1_HUMAN)
Ubiquitination at Lys280 (NX_P28562 [NX_P28562-1])
Modification sites at PhosphoSitePlus
- Protein Refseq
- NP_004408.1
- Function
- Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "DUSP1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3320z | Mouse Anti-DUSP1 Recombinant Antibody (clone 31A1) | WB, ELISA, ICC, IF | Mouse IgG2b, κ |
| MRO-0498-CN | Rabbit Anti-DUSP1 Recombinant Antibody (clone CBACN-202) | WB, IHC, IP, FC | Rabbit IgG |
| VS3-CJ846 | Rabbit Anti-DUSP1 Recombinant Antibody (VS3-CJ846) | WB, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0219 | APC-A*02:01/Human CL100 (QQFPGTAPKL) MHC Tetramer | FCM | |
| MHC-CN0220 | PE-A*02:01/Human CL100 (QQFPGTAPKL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2108 | Anti-DUSP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2109 | Anti-Mouse DUSP1 Immunohistochemistry Kit | IHC |
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