Recombinant Mouse Anti-EBV gH/gL Antibody (CL40) (CAT#: PABC-419)

This product is a recombinant Mouse antibody that can recognize EBV gH/gL. CL40 can block membrane fusion with both B cells and epithelial cells. CL40 binding to gHgL/gp42 complexes is not blocked by gp42 and does not interfere with gp42 binding to HLA class II, indicating that its ability to block membrane fusion with B cells represents a defect in gB activation. CL40 binds to an epitope in gH D-II that overlaps substantially with a region observed to interact with the gp42 HP.

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SDS-PAGE

Figure 1 Recombinant Mouse Anti-EBV gH/gL Antibody (CL40) (PABC-419) in SDS-PAGE

Figure 1 Recombinant Mouse Anti-EBV gH/gL Antibody (CL40) (PABC-419) in SDS-PAGE

SDS-PAGE analysis of PABC-419 in non-reduced (Lane 2) and reduced (Lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

SEC-HPLC

Figure 2 Recombinant Mouse Anti-EBV gH/gL Antibody (CL40) (PABC-419) in SEC-HPLC

Figure 2 Recombinant Mouse Anti-EBV gH/gL Antibody (CL40) (PABC-419) in SEC-HPLC

SEC-HPLC analysis of PABC-419 shows that the purity is more than 95%

WB

Figure 3 Recombinant Mouse Anti-EBV gH/gL Antibody (CL40) (PABC-419) in WB

Figure 3 Recombinant Mouse Anti-EBV gH/gL Antibody (CL40) (PABC-419) in WB

Western blot analysis of PABC-419 was performed with Epstein-Barr virus Glycoprotein H & Glycoprotein L Heterodimer Protein, Twin-Strep&His Tag.
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)
The secondary antibody: HRP Goat Anti-Mouse IgG (H+L)

DB

Figure 4 Recombinant Mouse Anti-EBV gH/gL Antibody (CL40) (PABC-419) in Dot Blot

Figure 4 Recombinant Mouse Anti-EBV gH/gL Antibody (CL40) (PABC-419) in Dot Blot

Dot Blot analysis of PABC-419 was performed with Epstein-Barr virus Glycoprotein H & Glycoprotein L Heterodimer Protein, Twin-Strep&His Tag.
The secondary antibody: HRP Goat Anti-Mouse IgG (H+L)

SDS-PAGE

Figure 5 Anti-EBV gH/gL Antibody (PABC-419) in SDS-PAGE

Figure 5 Anti-EBV gH/gL Antibody (PABC-419) in SDS-PAGE

SDS-PAGE analysis of PABC-419 in β-mercaptoethanol-reduced (Lane 1, 1.5 μg) and non-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

WB

Figure 6 Anti-EBV gH/gL Antibody (PABC-419) in WB

Figure 6 Anti-EBV gH/gL Antibody (PABC-419) in WB

WB analysis of PABC-419 was performed by coating with Epstein-Barr virus (EBV) (strain B95-8) gH & gL Heterodimer Protein (His & Flag Tag).

ELISA

Figure 7 Anti-EBV gH/gL Antibody (PABC-419) in ELISA

Figure 7 Anti-EBV gH/gL Antibody (PABC-419) in ELISA

ELISA analysis of PABC-419 was performed by coating with Epstein-Barr virus (EBV) (strain B95-8) gH & gL Heterodimer Protein (His & Flag Tag).


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • EBV gH/gL
  • Species Reactivity
  • EBV
  • Clone
  • CL40
  • Applications
  • ELISA, Inhib, FC, Neut

Product Property

  • Purity
  • >95% as determined by analysis by SDS-PAGE
  • Storage
  • Store at -20°C for long-term storage. Avoid freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Inhibition, Flow Cytometry and Neutralization. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.

Target

  • Alternative Names
  • EBV gH/gL; Envelope glycoprotein H; Envelope glycoprotein L

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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PABC-311 Recombinant Human Anti-EBV gH/gL Antibody (AMMO1) ELISA, BLI, Neut Human IgG
PABC-523 Mouse Anti-EBV gH/gL Recombinant Antibody (clone CL59) ELISA, FC, Neut, Inhib Mouse IgG1
PABC-524 Recombinant Mouse Anti-EBV gH/gL Antibody (E1D1) ELISA, FC, Neut, Inhib Mouse IgG2a, κ

Customer Reviews and Q&As

Customer Review Q&As

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  • User-Friendly Procedure
    The product had an easy-to-use protocol that makes learning how to utilize it efficiently simple. For someone who is unfamiliar with this particular antibody, this is a huge help.
  • Sturdy and Robust
    During extended storage, this antibody fragment showed to be robust and stable. I saw no deterioration even after using it for several months.
  • Enhanced Intricacy and Precision
    The affinity-purified nature increased the trustworthiness of the results in a variety of applications by enabling strong and accurate binding.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# PABC-419, RRID: AB_3111590)

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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