Recombinant Mouse Anti-EBV gH/gL Antibody (CL40) (CAT#: PABC-419)
This product is a recombinant Mouse antibody that can recognize EBV gH/gL. CL40 can block membrane fusion with both B cells and epithelial cells. CL40 binding to gHgL/gp42 complexes is not blocked by gp42 and does not interfere with gp42 binding to HLA class II, indicating that its ability to block membrane fusion with B cells represents a defect in gB activation. CL40 binds to an epitope in gH D-II that overlaps substantially with a region observed to interact with the gp42 HP.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
SDS-PAGE
Figure 1 Recombinant Mouse Anti-EBV gH/gL Antibody (CL40) (PABC-419) in SDS-PAGE
SDS-PAGE analysis of PABC-419 in non-reduced (Lane 2) and reduced (Lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 2 Recombinant Mouse Anti-EBV gH/gL Antibody (CL40) (PABC-419) in SEC-HPLC
SEC-HPLC analysis of PABC-419 shows that the purity is more than 95%
WB
Figure 3 Recombinant Mouse Anti-EBV gH/gL Antibody (CL40) (PABC-419) in WB
Western blot analysis of PABC-419 was performed with Epstein-Barr virus Glycoprotein H & Glycoprotein L Heterodimer Protein, Twin-Strep&His Tag.
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)
The secondary antibody: HRP Goat Anti-Mouse IgG (H+L)
DB
Figure 4 Recombinant Mouse Anti-EBV gH/gL Antibody (CL40) (PABC-419) in Dot Blot
Dot Blot analysis of PABC-419 was performed with Epstein-Barr virus Glycoprotein H & Glycoprotein L Heterodimer Protein, Twin-Strep&His Tag.
The secondary antibody: HRP Goat Anti-Mouse IgG (H+L)
SDS-PAGE
Figure 5 Anti-EBV gH/gL Antibody (PABC-419) in SDS-PAGE
SDS-PAGE analysis of PABC-419 in β-mercaptoethanol-reduced (Lane 1, 1.5 μg) and non-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
WB
Figure 6 Anti-EBV gH/gL Antibody (PABC-419) in WB
WB analysis of PABC-419 was performed by coating with Epstein-Barr virus (EBV) (strain B95-8) gH & gL Heterodimer Protein (His & Flag Tag).
ELISA
Figure 7 Anti-EBV gH/gL Antibody (PABC-419) in ELISA
ELISA analysis of PABC-419 was performed by coating with Epstein-Barr virus (EBV) (strain B95-8) gH & gL Heterodimer Protein (His & Flag Tag).
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- EBV gH/gL
- Species Reactivity
- EBV
- Clone
- CL40
- Applications
- ELISA, Inhib, FC, Neut
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Inhibition, Flow Cytometry and Neutralization. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
- Alternative Names
- EBV gH/gL; Envelope glycoprotein H; Envelope glycoprotein L
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Fab Antibody Products
ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-311 | Recombinant Human Anti-EBV gH/gL Antibody (AMMO1) | ELISA, BLI, Neut | Human IgG |
| PABC-523 | Mouse Anti-EBV gH/gL Recombinant Antibody (clone CL59) | ELISA, FC, Neut, Inhib | Mouse IgG1 |
| PABC-524 | Recombinant Mouse Anti-EBV gH/gL Antibody (E1D1) | ELISA, FC, Neut, Inhib | Mouse IgG2a, κ |
Customer Reviews and Q&As
Customer Review
Q&As
User-Friendly Procedure
The product had an easy-to-use protocol that makes learning how to utilize it efficiently simple. For someone who is unfamiliar with this particular antibody, this is a huge help.
Sturdy and Robust
During extended storage, this antibody fragment showed to be robust and stable. I saw no deterioration even after using it for several months.
Enhanced Intricacy and Precision
The affinity-purified nature increased the trustworthiness of the results in a variety of applications by enabling strong and accurate binding.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABC-419, RRID: AB_3111590)
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For Research Use Only. Not For Clinical Use.
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