CytoStream™ Rabbit Anti-EHD1 Recombinant Antibody (VS13-YC343)
CAT#: VS13-YC343
The CytoStream™ Rabbit Anti-EHD1 Recombinant Antibody is specific for human, mouse and rat EHD1. EHD1, related to EH domain proteins, affects endocytosis processes, participating in receptor sorting pathways that modulate signaling routes and intracellular transport. The anti-EHD1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67747/1568_E12_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66751/1667_E12_6_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49890/128359_A_7_3.jpg
Normal Tissue
Figure 4 Testis
Elongated or late spermatids Staining: Low Intensity: Moderate Quantity: <25% Peritubular cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49890/128359_A_5_6.jpg
Normal Tissue
Figure 5 Placenta
Trophoblastic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49890/128359_A_1_7.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110047-EHD1
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Specifications
- Immunogen
- Recombinant human EHD1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat EHD1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Conjugate
- NONE
- Related Disease
- Cardiovascular Disease
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 150 mM NaCl and 50% Glycerol.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/50-1/100
Immunofluorescence (IF): 1/50-1/200
Immunoprecipitation (IP): 1/50
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- EH Domain Containing 1; Testilin; PAST Homolog 1; HPAST1; PAST1; PAST; EH Domain-Containing Protein 1; H-PAST;
- Gene ID
- 10938
- UniProt ID
- Q9H4M9
- Long Name
- EH Domain Containing 1
- Sequence Similarities
- Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. EHD subfamily.
- Cellular Localization
- Cell membrane; Cell projection; Cilium; Endosome; Membrane
- Protein Refseq
- NP_001269373.1; NP_001269374.1; NP_006786.2
- Function
- Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes.
Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth (By similarity).
Plays a role in myoblast fusion (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "EHD1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1106 | Hi-Affi™ Rabbit Anti-EHD1 Recombinant Antibody (clone DS1106AB) | WB, IP, FC, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY1706 | Rabbit Anti-EHD1 Recombinant Antibody (clone R01-4E6) | WB, IHC-Fr, IHC-P, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2192 | Anti-EHD1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2193 | Anti-Mouse EHD1 Immunohistochemistry Kit | IHC |
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