CytoStream™ Rabbit Anti-EHD1 Recombinant Antibody (VS13-YC343) (CAT#: VS13-YC343)
The CytoStream™ Rabbit Anti-EHD1 Recombinant Antibody is specific for human, mouse and rat EHD1. EHD1, related to EH domain proteins, affects endocytosis processes, participating in receptor sorting pathways that modulate signaling routes and intracellular transport. The anti-EHD1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67747/1568_E12_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67747/1568_E12_2_selected.jpg
Figure 2 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66751/1667_E12_6_selected.jpg
(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66751/1667_E12_6_selected.jpg
Figure 3 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49890/128359_A_7_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49890/128359_A_7_3.jpg
Figure 4 Testis
(Elongated or late spermatids Staining: Low Intensity: Moderate Quantity: <25% Peritubular cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49890/128359_A_5_6.jpg
(Elongated or late spermatids Staining: Low Intensity: Moderate Quantity: <25% Peritubular cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49890/128359_A_5_6.jpg
Figure 5 Placenta
(Trophoblastic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49890/128359_A_1_7.jpg
(Trophoblastic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49890/128359_A_1_7.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110047-EHD1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110047-EHD1
Specifications
- Immunogen
- Recombinant human EHD1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat EHD1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Conjugate
- NONE
- Related Disease
- Cardiovascular Disease
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 150 mM NaCl and 50% Glycerol.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/50-1/100
Immunofluorescence (IF): 1/50-1/200
Immunoprecipitation (IP): 1/50
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- EH Domain Containing 1; Testilin; PAST Homolog 1; HPAST1; PAST1; PAST; EH Domain-Containing Protein 1; H-PAST;
- Gene ID
- 10938
- UniProt ID
- Q9H4M9
- Long Name
- EH Domain Containing 1
- Sequence Similarities
- Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. EHD subfamily.
- Cellular Localization
- Cell membrane; Cell projection; Cilium; Endosome; Membrane
- Protein Refseq
- NP_001269373.1; NP_001269374.1; NP_006786.2
- Function
- Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes.
Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth (By similarity).
Plays a role in myoblast fusion (By similarity).
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1106 | Hi-Affi™ Rabbit Anti-EHD1 Recombinant Antibody (clone DS1106AB) | WB, IP, FC, ICC, IF | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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