AibGenesis™ Rabbit Anti-EIF1AX Recombinant Antibody (AibG-Rb-AX131)
CAT#: VS14-YC331
Leveraging our deep learning platform, the anti-EIF1AX mAb (AibG-Rb-AX131) is crafted for high-affinity recognition of EIF1AX in human, mouse, and rat. EIF1AX is an essential translation initiation factor that facilitates the assembly of the 43S pre-initiation complex and scanning of the mRNA leader. It is a key player in the translational control pathway, and mutations in this factor are linked to various human cancers.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2561/if_selected.jpg
Normal Tissue
Figure 2 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2561/22239_A_4_6.jpg
Normal Tissue
Figure 3 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2561/22239_A_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2561/22239_A_8_3.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2561/22239_A_8_8.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2561/22239_A_9_4.jpg
Normal Tissue
Figure 7 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2561/22337_B_9_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000173674-EIF1AX
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- EIF1AX
- Species Reactivity
- Human, Mouse, Rat
- Clone
- AibG-Rb-AX131
- Applications
- Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC)
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- Western Blot (WB): 1/500-1/2000
Immunohistochemistry (IHC): 1/50-1/200
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunoprecipitation (IP): 1/50
Flow Cytometry (FC): 1/50
Target
- Alternative Names
- Eukaryotic Translation Initiation Factor 1A, X-Linked; Eukaryotic Translation Initiation Factor 1A, X Chromosome; Eukaryotic Translation Initiation Factor 4C; EIF-1A X Isoform; EIF-4C; EIF1A;
- Gene ID
- 1964
- UniProt ID
- P47813
- Long Name
- Eukaryotic Translation Initiation Factor 1A, X-Linked
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "EIF1AX"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1111 | Hi-Affi™ Rabbit Anti-EIF1AX Recombinant Antibody (clone DS1111AB) | ICC, IF, IP, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY480 | Recombinant Rabbit Anti-EIF1AX Antibody (clone R04-8B7) | WB, IHC-F, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2201 | Anti-EIF1AX Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2202 | Anti-Mouse EIF1AX Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0426-FY390 | AibGenesis™ Rabbit Anti-EIF1AX Recombinant Antibody (AibG-Rb-EI390) | WB, IHC, ICC, IF, FC, IP | Rabbit IgG |
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