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EIF2AK2
Rabbit Anti-EIF2AK2 (pT451) Recombinant Antibody (clone R08-2G5)

Rabbit Anti-EIF2AK2 (pT451) Recombinant Antibody (clone R08-2G5) (CAT#: VS3-WK2010)

Name: Rabbit Anti-EIF2AK2 (pT451) Recombinant Antibody (clone R08-2G5)
SKU: VS3-WK2010
Rating: 5 (1 reviews)

This product is a rabbit recombinant antibody that is specific to phosphorylated EIF2AK2. The target of this antibody is phospho-EIF2AK2 (Thr451). The clone R08-2G5 can be used in WB analysis at a dilution of 1/500-1/1000. The molecular weight of EIF2AK2 (pThr451) observed in WB experiments using this antibody is 74 kDa.

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1 Rabbit Anti-EIF2AK2 (pThr451) Recombinant Antibody (clone R08-2G5) in WB.

Figure 1 Rabbit Anti-EIF2AK2 (pThr451) Recombinant Antibody (clone R08-2G5) in WB.

Western blot analysis of EIF2AK2 (phospho-Thr451) in Hela, Hela+CalyculinA lysates using anti-EIF2AK2 (pThr451) antibody, Cat# VS3-WK2010.

Subcellular Location

Figure 1 IF staining of human cell line SK-MEL-30
(Immunofluorescent staining of human cell line SK-MEL-30 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63893/if_selected.jpg

Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3845/if_selected.jpg

Normal Tissue

Figure 3 Cerebral cortex
(Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3845/10337_B_8_5.jpg

Figure 4 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3845/10337_A_8_3.jpg

Figure 5 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3845/10337_A_8_5.jpg

Figure 6 Testis
(Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3845/10337_A_6_6.jpg

Figure 7 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3845/10337_A_9_8.jpg

RNA Expression

Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000055332-EIF2AK2

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
A synthetic phosphopeptide corresponding to residues Thr451 of human PKR

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Phospho-Thr451 of human EIF2AK2

Species Reactivity
Human

Clone
R08-2G5

Applications
WB

Conjugate
Unconjugated

Product Property

Clonality
Monoclonal

Purification
Protein A affinity purified

Purity
>95% as determined by SDS-PAGE

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
WB: 1/500-1/1000

Target

Alternative Names
Eukaryotic Translation Initiation Factor 2 Alpha Kinase 2; Protein Kinase, Interferon-Inducible Double Stranded RNA Dependent; Protein Phosphatase 1, Regulatory Subunit 83; Tyrosine-Protein Kinase EIF2AK2; P1/EIF-2A Protein Kinase; EIF-2A Protein Kinase 2; Protein Kinase R; P68 Kinase; PRKR; PKR; Interferon-Induced, Double-Stranded RNA-Activated Protein Kinase

Gene ID
5610

UniProt ID
P19525

Long Name
Eukaryotic Translation Initiation Factor 2 Alpha Kinase 2

Isotype Control

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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MOB-2694z	Mouse Anti-EIF2AK2 Recombinant Antibody (clone 21F8)	WB, ELISA, FC, IHC	Mouse IgG1
MRO-1242-CN	Rabbit Anti-EIF2AK2 Recombinant Antibody (clone CBACN-453)	WB, IF, IHC, IP	Rabbit IgG
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