Rabbit Anti-EIF2AK2 Recombinant Antibody (clone 2F8)
CAT#: ZG-0646J
This product is a rabbit antibody that recognizes EIF2AK2.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-EIF2AK2 Antibody (ZG-0646J) in WB
Western Blot
Positive WB detected in Hela whole cell lysate(treated with Calyculin A or not)
All lanes Phospho-EIF2AK2 antibody at 1.25μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 62 KDa
Observed band size: 62 KDa
IHC
Figure 2 Rabbit Anti-EIF2AK2 Antibody (ZG-0646J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 3 Rabbit Anti-EIF2AK2 Antibody (ZG-0646J) in IF
Immunofluorescence staining of Hela cells(treated with 50mM Calyculin A for 30min) with this product at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
❮
❯
Subcellular Location
Figure 1 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63893/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3845/if_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3845/10337_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3845/10337_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3845/10337_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3845/10337_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3845/10337_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000055332-EIF2AK2
❮
❯
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-EIF2AK2 (T446)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human EIF2AK2
- Species Reactivity
- Human
- Clone
- 2F8
- Applications
- ELISA, WB, IHC, IF
- Conjugate
- Uconjugated
- Related Disease
- Infections
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- PKR; PRKR; LEUDEN; EIF2AK1; PPP1R83; interferon-induced, double-stranded RNA-activated protein kinase; P1/eIF-2A protein kinase; double stranded RNA activated protein kinase; eIF-2A protein kinase 2; interferon-inducible elF2alpha kinase; p68 kinase; protein kinase R; protein kinase, interferon-inducible double stranded RNA dependent; protein phosphatase 1, regulatory subunit 83; tyrosine-protein kinase EIF2AK2
- Gene ID
- 5610
- UniProt ID
- P19525
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for ZG-0646J. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "EIF2AK2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0669MZ | Chicken Anti-EIF2AK2 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1114 | Hi-Affi™ Rabbit Anti-EIF2AK2 Recombinant Antibody (clone DS1114AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1242-CN | Rabbit Anti-EIF2AK2 Recombinant Antibody (clone CBACN-453) | WB, IF, IHC, IP | Rabbit IgG |
| VS3-QX355 | Mouse Anti-EIF2AK2 Recombinant Antibody (clone 7E7-F11-G6) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-QX356 | Mouse Anti-EIF2AK2 Recombinant Antibody (clone 3A-H10) | WB, IHC, FC | Mouse IgG1 |
| VS3-QX357 | Mouse Anti-EIF2AK2 Recombinant Antibody (clone 1441CT628.33.40) | WB, ICC | Mouse IgG1 |
| VS3-CJ749 | Mouse Anti-EIF2AK2 Recombinant Antibody (VS3-CJ749) | WB, IF | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY85 | AbPlus™ Anti-EIF2AK2 Magnetic Beads (CBACN-453) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY739 | Anti-EIF2AK2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2205 | Anti-Human EIF2AK2 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, ELISA, FuncS
Application: WB, ELISA, FC, IHC, IP
Application: Neut, FC
Application: SPR, Inhib, FuncS
Application: ELISA, IF, Block, FuncS
Application: ELISA, FC, Neut, Inhib
-3.jpg)
Application: IB, FC, ELISA, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
