Rabbit Anti-EIF2AK2 Recombinant Antibody (clone 2F8) (CAT#: ZG-0646J)
This product is a rabbit antibody that recognizes EIF2AK2.
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Custom Production
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WB
Figure 1 Rabbit Anti-EIF2AK2 Antibody (ZG-0646J) in WB
Western Blot
Positive WB detected in Hela whole cell lysate(treated with Calyculin A or not)
All lanes Phospho-EIF2AK2 antibody at 1.25μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 62 KDa
Observed band size: 62 KDa
IHC
Figure 2 Rabbit Anti-EIF2AK2 Antibody (ZG-0646J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 3 Rabbit Anti-EIF2AK2 Antibody (ZG-0646J) in IF
Immunofluorescence staining of Hela cells(treated with 50mM Calyculin A for 30min) with this product at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-EIF2AK2 (T446)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human EIF2AK2
- Species Reactivity
- Human
- Clone
- 2F8
- Applications
- ELISA, WB, IHC, IF
- Conjugate
- Uconjugated
- Related Disease
- Infections
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- PKR; PRKR; LEUDEN; EIF2AK1; PPP1R83; interferon-induced, double-stranded RNA-activated protein kinase; P1/eIF-2A protein kinase; double stranded RNA activated protein kinase; eIF-2A protein kinase 2; interferon-inducible elF2alpha kinase; p68 kinase; protein kinase R; protein kinase, interferon-inducible double stranded RNA dependent; protein phosphatase 1, regulatory subunit 83; tyrosine-protein kinase EIF2AK2
- Gene ID
- 5610
- UniProt ID
- P19525
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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