CytoStream™ Rabbit Anti-EIF2AK4 Recombinant Antibody (VS13-YC347)
CAT#: VS13-YC347
The CytoStream™ Rabbit Anti-EIF2AK4 Recombinant Antibody is specific for human EIF2AK4. EIF2AK4 participates in translational control under nutrient deprivation, phosphorylating EIF2 protein subunits and modulating cellular responses to stress conditions. The anti-EIF2AK4 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11811/110_C8_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human gallbladder
Immunohistochemical staining of human gallbladder shows weak cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11811/28186_A_5_4_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11811/28186_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11811/28186_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11811/28186_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11811/28186_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11811/28186_A_9_8.jpg
Normal Tissue
Figure 8 Tonsil
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Squamous epithelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11811/28186_A_5_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000128829-EIF2AK4
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Specifications
- Immunogen
- Recombinant human GCN2, aa 1-50.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human EIF2AK4
- Species Reactivity
- Human
- Applications
- WB, ICC, IF, IHC-P, FC
- Conjugate
- NONE
- Related Disease
- Autosomal recessive pulmonary venoocclusive disease
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Eukaryotic Translation Initiation Factor 2 Alpha Kinase 4; GCN2-Like Protein; GCN2; Eukaryotic Translation Initiation Factor 2-Alpha Kinase 4; General Control Nonderepressible 2; EIF-2-Alpha Kinase GCN2;
- Gene ID
- 440275
- UniProt ID
- Q9P2K8
- Long Name
- Eukaryotic Translation Initiation Factor 2 Alpha Kinase 4
- Cellular Localization
- Cytoplasm
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "EIF2AK4"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0753MZ | Chicken Anti-GCN2 Polyclonal IgY | ELISA, IF, IHC, IP, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1115 | Hi-Affi™ Rabbit Anti-EIF2AK4 Recombinant Antibody (clone DS1115AB) | IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0642-CN | Rabbit Anti-EIF2AK4 Recombinant Antibody (clone JA03-83) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-WK2097 | Rabbit Anti-EIF2AK4 (pT899) Recombinant Antibody (clone R08-4N7) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2207 | Anti-EIF2AK4 Immunohistochemistry Kit | IHC |
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