CytoStream™ Rabbit Anti-EIF4E Recombinant Antibody (VS13-YC350)
CAT#: VS13-YC350
The CytoStream™ Rabbit Anti-EIF4E Recombinant Antibody is specific for human and mouse EIF4E. EIF4E, part of the translation initiation complex, aids ribosomal interactions with mRNA caps, serving proto-oncogenic functions linked to transformative cellular processes. The anti-EIF4E antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/500-1/1000).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & cytoplasmic bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68358/1607_H9_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16316/951_E2_2_selected.jpg
Normal Tissue
Figure 3 Testis
Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4077/11592_A_6_6.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4077/11592_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4077/11592_A_8_5.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000151247-EIF4E
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Specifications
- Immunogen
- Recombinant eIF4E.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse EIF4E
- Species Reactivity
- Human, Mouse
- Applications
- WB, IHC-P, ICC, IF, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500
Immunohistochemistry-paraffin (IHC-P): 1/200
Immunocytochemistry (ICC): 1/50
Flow cytometry (FC): 1/500-1/1000
Target
- Alternative Names
- Eukaryotic Translation Initiation Factor 4E; MRNA Cap-Binding Protein; EIF-4F 25 KDa Subunit; EIF4EL1; EIF-4E; EIF4F;
- Gene ID
- 1977
- UniProt ID
- P06730
- Long Name
- Eukaryotic Translation Initiation Factor 4E
- Sequence Similarities
- Belongs to the eukaryotic initiation factor 4E family.
- Cellular Localization
- Cytoplasm; Nucleus
- Protein Refseq
- NP_001124150.1; NP_001124151.1; NP_001317946.1
- Function
- Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis (By similarity).
In P-bodies, component of a complex that mediates the storage of translationally inactive mRNAs in the cytoplasm and prevents their degradation (PubMed:24335285).
May play an important role in spermatogenesis through translational regulation of stage-specific mRNAs during germ cell development (By similarity).
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Related Signaling Pathways
Insulin Signaling Pathway
MAPK Signaling Pathway
mTOR Signaling Pathway
Related Diseases
Insulin Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "EIF4E"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2681z | Mouse Anti-EIF4E Recombinant Antibody (clone 27G7) | WB, ELISA, ICC, IF, IHC | Mouse IgG1 |
| ZG-0648J | Rabbit Anti-EIF4E Recombinant Antibody (clone 1F3) | ELISA, WB, IHC | Rabbit IgG |
| VS3-QX360 | Mouse Anti-EIF4E Recombinant Antibody (clone 8C8-C10-C12) | WB | Mouse IgG1 |
| VS3-QX361 | Mouse Anti-EIF4E Recombinant Antibody (clone 163CT48.1.9) | WB, ICC | Mouse IgG1, κ |
| VS7-HM850 | Mouse Anti-EIF4E Recombinant Antibody (clone 5D11) | WB, IHC, ICC, FCM | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1128 | Hi-Affi™ Rabbit Anti-EIF4E Recombinant Antibody (clone DS1128AB) | WB, IP, IHC-P, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY752 | Anti-EIF4E Immunohistochemistry Kit | IHC | |
| VS-0525-XY2229 | Anti-Mouse EIF4E Immunohistochemistry Kit | IHC | |
| VS-0525-XY2228 | Anti-Human EIF4E Immunohistochemistry Kit | IHC |
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