Rabbit Anti-EIF4E Recombinant Antibody (clone 1F3)
CAT#: ZG-0648J
This product is a rabbit antibody that recognizes EIF4E.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-EIF4E Antibody (ZG-0648J) in WB
Western Blot
Positive WB detected in 293 whole cell lysate,HepG2 whole cell lysate(treated with Calyculin A or not)
All lanes Phospho-EIF4E antibody at 1.2μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25 KDa
Observed band size: 25 KDa
IHC
Figure 2 Rabbit Anti-EIF4E Antibody (ZG-0648J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & cytoplasmic bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68358/1607_H9_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16316/951_E2_2_selected.jpg
Normal Tissue
Figure 3 Testis
Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4077/11592_A_6_6.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4077/11592_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4077/11592_A_8_5.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000151247-EIF4E
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Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-EIF4E (S209)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human EIF4E
- Species Reactivity
- Human
- Clone
- 1F3
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
- Related Disease
- Tumor
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
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Related Signaling Pathways
Insulin Signaling Pathway
MAPK Signaling Pathway
mTOR Signaling Pathway
Related Diseases
Insulin Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "EIF4E"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1128 | Hi-Affi™ Rabbit Anti-EIF4E Recombinant Antibody (clone DS1128AB) | WB, IP, IHC-P, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1838-CN | Rabbit Anti-EIF4E Polyclonal Antibody (MRO-1838-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-QX360 | Mouse Anti-EIF4E Recombinant Antibody (clone 8C8-C10-C12) | WB | Mouse IgG1 |
| VS3-QX361 | Mouse Anti-EIF4E Recombinant Antibody (clone 163CT48.1.9) | WB, ICC | Mouse IgG1, κ |
| VS7-HM850 | Mouse Anti-EIF4E Recombinant Antibody (clone 5D11) | WB, IHC, ICC, FCM | Mouse IgG1 |
| VS7-HM851 | Mouse Anti-EIF4E Recombinant Antibody (clone CBL221HM) | WB, IHC-p, IF, ICC, FCM, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY752 | Anti-EIF4E Immunohistochemistry Kit | IHC | |
| VS-0525-XY2229 | Anti-Mouse EIF4E Immunohistochemistry Kit | IHC | |
| VS-0525-XY2228 | Anti-Human EIF4E Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC350 | CytoStream™ Rabbit Anti-EIF4E Recombinant Antibody (VS13-YC350) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
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