Rabbit Anti-EIF4E Recombinant Antibody (clone 1F3) (CAT#: ZG-0648J)
This product is a rabbit antibody that recognizes EIF4E.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-EIF4E Antibody (ZG-0648J) in WB
Western Blot
Positive WB detected in 293 whole cell lysate,HepG2 whole cell lysate(treated with Calyculin A or not)
All lanes Phospho-EIF4E antibody at 1.2μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25 KDa
Observed band size: 25 KDa
IHC
Figure 2 Rabbit Anti-EIF4E Antibody (ZG-0648J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-EIF4E (S209)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human EIF4E
- Species Reactivity
- Human
- Clone
- 1F3
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
- Related Disease
- Tumor
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- CBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1; eukaryotic translation initiation factor 4E; eIF-4F 25 kDa subunit; eukaryotic translation initiation factor 4E-like 1; mRNA cap-binding protein
- Gene ID
- 1977
- UniProt ID
- P06730
Related Resources
Insulin Signaling Pathway
MAPK Signaling Pathway
mTOR Signaling Pathway
Insulin Resistance
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2681z | Mouse Anti-EIF4E Recombinant Antibody (clone 27G7) | WB, ELISA, ICC, IF, IHC | Mouse IgG1 |
| VS3-CJ444 | Mouse Anti-EIF4E Recombinant Antibody (VS3-CJ444) | WB, ICC | Mouse IgG |
| VS3-FY489 | Recombinant Rabbit Anti-EIF4E Antibody (clone R04-4H7) | WB, IHC-P, IP | Rabbit IgG |
| VS3-FY490 | Recombinant Rabbit Anti-EIF4E Antibody (clone R04-5A4) | WB, IHC-F, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS3-FY491 | Recombinant Rabbit Anti-EIF4E Antibody (clone R08-1C5) | WB, ICC, IF, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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