Rabbit Anti-ELAVL1 Recombinant Antibody (clone R04-3A6) (CAT#: VS3-XY3343)
This antibody is a recombinant rabbit antibody binding with ELAVL1 (ELAV like RNA binding protein 1), which involves in embryonic stem cell (ESC) differentiation. It reacts with human, mouse and rat ELAVL1. It has been detected using Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5256/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5256/if_selected.jpg
Figure 2 IHC staining of human parathyroid gland
(Immunohistochemical staining of human parathyroid gland shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5256/ihc_selected.jpg
(Immunohistochemical staining of human parathyroid gland shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5256/ihc_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5256/13674_B_7_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5256/13674_B_7_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5256/13674_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5256/13674_A_7_3.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Cells in tubules Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5256/13674_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Cells in tubules Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5256/13674_A_8_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5256/13674_A_4_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5256/13674_A_4_6.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000066044-ELAVL1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000066044-ELAVL1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ELAVL1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-3A6
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Related Disease
- Colorectal Cancer; Ureteral Obstruction
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- HUR; Hua; MelG; ELAV1
- Gene ID
- 1994
- UniProt ID
- Q15717
- Long Name
- ELAV like RNA binding protein 1
- Cellular Localization
- Nucleus, Cytoplasm, Stress granule
- Post Translation Modifications
- Phosphorylated by MAPKAPK2 (PubMed:14517288).
Phosphorylated by PRKCD (PubMed:18285462).
(Q15717-ELAV1_HUMAN)
Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge. (Q15717-ELAV1_HUMAN)
Ubiquitination at Lys50, Lys55, Lys72, Lys104, Lys120, Lys191, Lys313, and Lys320 (NX_Q15717 [NX_Q15717-1])
Modification sites at PhosphoSitePlus (Q15717)
Glycosylation from GlyGen (Q15717) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_047294339.1; XP_047294340.1
- Function
- RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (PubMed:14517288, 18285462, 31358969).
Involved in embryonic stem cell (ESC) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESC differentiation.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1825MZ | Recombinant Mouse Anti-Human ELAVL1 Antibody | WB | Mouse antibody |
| MRO-0803-CN | Rabbit Anti-ELAVL1 Recombinant Antibody (clone JM96-33) | WB, IHC, IP, FC | Rabbit IgG |
| VS3-WK995 | Mouse Anti-ELAVL1 Recombinant Antibody (VS3-WK995) | WB, ICC | Mouse IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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