Mouse Anti-ENO2 Recombinant Antibody (clone 13E2) (CAT#: MOB-0145F)

Figure 1 Mouse Anti-ENO2 Recombinant Antibody (clone 13E2) (MOB-0145F) in IF

Immunofluorescence analysis of Hela cells. 1. Cdk2 polyclonal antibody (red) was diluted at 1:200 (overnight at 4°C). NSE monoclonal antibody (13E2) (green) was diluted at 1:200 (overnight at 4°C). 2. Goat anti-rabbit Alexa Fluor 594 diluted 1:1000 (room temperature, 50 minutes). Goat Anti Mouse Alexa Fluor 488 is diluted 1:1000 (room temperature, 50 minutes).

Figure 2 Mouse Anti-ENO2 Recombinant Antibody (clone 13E2) (MOB-0145F) in IHC

Immunohistochemical analysis of paraffin-embedded human colon tissue. 1. The antibody is diluted 1:200 (overnight at 4°C). 2. Sodium citrate pH 6.0 is used for antibody repair (>98°C, 20 minutes). 3. Dilute the secondary antibody at 1:200 (room temperature, 30 minutes). The negative control is only used by the secondary antibody.

Figure 3 Mouse Anti-ENO2 Recombinant Antibody (clone 13E2) (MOB-0145F) in IHC

Immunohistochemical analysis of paraffin-embedded rat heart tissue. 1. The antibody is diluted 1:200 (overnight at 4°C). 2. Sodium citrate pH 6.0 is used for antibody repair (>98°C, 20 minutes). 3. Dilute the secondary antibody at 1:200 (room temperature, 30 minutes). The negative control is only used by the secondary antibody.

Figure 4 Mouse Anti-ENO2 Recombinant Antibody (clone 13E2) (MOB-0145F) in IF

Immunofluorescence analysis of human appendix tissue. 1. NSE monoclonal antibody (13E2) (red) is diluted at 1:200 (4°C, overnight). 2. The Cy3 labeled secondary antibody was diluted 1:300 (room temperature, 50 minutes). 3. Picture B: DAPI (blue) for 10 minutes. Figure A: Goal. Picture B: DAPI. Picture C: The merger of A+B.

Figure 5 Mouse Anti-ENO2 Recombinant Antibody (clone 13E2) (MOB-0145F) in WB

1) Western blot analysis of Hela, 2) Jurkat, 3) 293T cell lysates, diluted 1:3000.