Rabbit Anti-ERN1 Recombinant Antibody (clone 1F12) (CAT#: ZG-0655J)
This product is a rabbit antibody that recognizes ERN1.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-ERN1 Antibody (ZG-0655J) in WB
Western Blot
Positive WB detected in: 293 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate (treated with Calyculin A or EGF)
All lanes: Phospho-ERN1 antibody at 0.75μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 110 kDa
Observed band size: 110 kDa
IF
Figure 2 Rabbit Anti-ERN1 Antibody (ZG-0655J) in IF
Immunofluorescence staining of Hela cells with this product at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-ERN1 (S724)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ERN1
- Species Reactivity
- Human
- Clone
- 1F12
- Applications
- ELISA, WB, IF
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence.
WB:1:500-1:5000, IF:1:20-1:200
Target
- Alternative Names
- IRE1; IRE1P; IRE1a; hIRE1p; serine/threonine-protein kinase/endoribonuclease IRE1; ER to nucleus signalling 1; inositol-requiring 1; inositol-requiring enzyme 1; inositol-requiring protein 1; ire1-alpha; protein kinase/endoribonuclease
- Gene ID
- 2081
- UniProt ID
- O75460
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Chicken IgY Antibody Products
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0752MZ | Chicken Anti-IRE1A Polyclonal IgY | ELISA, IP, WB | Chicken antibody |
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For Research Use Only. Not For Clinical Use.
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