Rabbit Anti-F2 Recombinant Antibody (clone R08-5G1) (CAT#: VS3-FY1856)
The R08-5G1 recombinant monoclonal antibody is designed to recognize mouse F2. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-P. This prothrombin antibody was verified by western blot analysis of prothrombin in mouse liver, rat liver lysates.
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WB
Figure 1 Anti-F2 Antibody (VS3-FY1856) in WB.
The Prothrombin expression in mouse liver, rat liver lysates were detected using the anti-Prothrombin by western blot assay.
Specifications
- Immunogen
- Recombinant mouse prothrombin protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Mouse and Rat F2
- Species Reactivity
- Mouse, Rat
- Clone
- R08-5G1
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 70 kDa, while the observed molecular weight stands at 70 kDa.
- Related Disease
- Prothrombin Deficiency, Congenital and Prothrombin Deficiency.
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Coagulation Factor II, Thrombin; Prepro-Coagulation Factor II; EC 3.4.21.5; Coagulation Factor II (Thrombin); Coagulation Factor II; Prothrombin B-Chain; Serine Protease
- Gene ID
- 14061
- UniProt ID
- P19221
- Sequence Similarities
- Belongs to the peptidase S1 family.
- Post Translation Modifications
- The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
- Protein Refseq
- NP_034298.1
- Function
- Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-344-F(E) | Recombinant Anti-human F2 Antibody Fab Fragment | ELISA, IP, FuncS | Fab |
| MHH-344-F(E) | Recombinant Human Anti-human F2 Antibody Fab Fragment | ELISA, WB, Dot, FuncS | Fab |
| PFBL-092 | Human Anti-F2 Recombinant Antibody (clone YC045); Fab Fragment | ELISA, WB, FuncS | Human Fab |
| HPAB-0049-YC-F(E) | Human Anti-F2 Recombinant Antibody (clone h1601-008); Fab Fragment | ELISA | Humanized Fab |
| HPAB-0050-YC-F(E) | Mouse Anti-F2 Recombinant Antibody (clone aTM-1); Fab Fragment | ELISA | Mouse Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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