Rabbit Anti-F2 Recombinant Antibody (clone R08-5G1)
CAT#: VS3-FY1856
The R08-5G1 recombinant monoclonal antibody is designed to recognize mouse F2. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-P. This prothrombin antibody was verified by western blot analysis of prothrombin in mouse liver, rat liver lysates.
Gene Expression
Tested Data
Normal Tissue
Figure 1 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51476/133725_A_8_4.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enriched (Hep G2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000180210-F2
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WB
Figure 1 Anti-F2 Antibody (VS3-FY1856) in WB.
The Prothrombin expression in mouse liver, rat liver lysates were detected using the anti-Prothrombin by western blot assay.
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Specifications
- Immunogen
- Recombinant mouse prothrombin protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Mouse and Rat F2
- Species Reactivity
- Mouse, Rat
- Clone
- R08-5G1
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 70 kDa, while the observed molecular weight stands at 70 kDa.
- Related Disease
- Prothrombin Deficiency, Congenital and Prothrombin Deficiency.
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Coagulation Factor II, Thrombin; Prepro-Coagulation Factor II; EC 3.4.21.5; Coagulation Factor II (Thrombin); Coagulation Factor II; Prothrombin B-Chain; Serine Protease
- Gene ID
- 14061
- UniProt ID
- P19221
- Sequence Similarities
- Belongs to the peptidase S1 family.
- Post Translation Modifications
- The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
- Protein Refseq
- NP_034298.1
- Function
- Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| HPAB-J0085-YC-S(P) | Human Anti-F2 Recombinant Antibody (clone AP021/YC); scFv Fragment | FuncS | Human scFv |
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| CAT | Product Name | Application | Type |
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| PABL-469 | Human Anti-F2 Recombinant Antibody (clone YC050) | WB, IF, FuncS | Human IgG |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2407 | Anti-F2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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