Mouse Anti-FASLG Recombinant Antibody (VS3-FY2700) (CAT#: VS3-FY2700)

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Fas ligand of human

• Species Reactivity
• Human

• Applications
• ICC, FC, ELISA

• Conjugate
• Unconjugated, FITC

• MW
• The molecular weight of the target protein is approximately 31.5 kDa.

• Related Disease
• Autoimmune Lymphoproliferative Syndrome and Immunodeficiency 98 With Autoinflammation, X-Linked

• Clonality
• Monoclonal Antibody

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Immunocytochemistry: 1/50-1/200
  Flow cytometry: 1/50-1/200
  ELISA: 1/1000-1/10000

• Alternative Names
• Fas Ligand; Fas Ligand (TNF Superfamily, Member 6); Apoptosis Antigen Ligand; Fas Antigen Ligand; CD95 Ligand; APT1LG1; TNFSF6; CD95-L; CD95L; APTL; FASL

• Gene ID
• 356

• Sequence Similarities
• Belongs to the tumor necrosis factor family.

• Cellular Localization
• Cell membrane; Single-pass type II membrane protein
  Cytoplasmic vesicle lumen
  Lysosome lumen

• Post Translation Modifications
• The soluble form derives from the membrane form by proteolytic processing.
  The membrane-bound form undergoes two successive intramembrane proteolytic cleavages.
  The first one is processed by ADAM10 producing an N-terminal fragment, which lacks the receptor-binding extracellular domain.
  This ADAM10-processed FasL (FasL APL) remnant form is still membrane anchored and further processed by SPPL2A that liberates the FasL intracellular domain (FasL ICD).
  FasL shedding by ADAM10 is a prerequisite for subsequent intramembrane cleavage by SPPL2A in T-cells.
  N-glycosylated.
  Glycosylation enhances apoptotic activity.
  Phosphorylated by FGR on tyrosine residues; this is required for ubiquitination and subsequent internalization.
  Monoubiquitinated.
  Glycosylation at Asn184, Asn250, and Asn260
  Modification sites at PhosphoSitePlus
  Glycosylation from GlyGen 3 sites

• Protein Refseq
• NP_000630.1; NP_001289675.1

• Function
• TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance (By similarity).
  Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis.

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

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