Rabbit Anti-FDFT1 Recombinant Antibody (clone R07-9J7)
CAT#: VS3-XY3356
This is a recombinant rabbit antibody targeting the human FDFT1 (Farnesyl-diphosphate farnesyltransferase 1), which catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene. The antibody also has cross-reactivity against mouse and rat FDFT1. It has been determined using Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8874/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in cortical cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8874/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8874/24809_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8874/24809_A_8_4.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8874/24809_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8874/24809_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Group enriched (Daudi, RPMI-8226)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000079459-FDFT1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat FDFT1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-9J7
- Applications
- WB, IHC-P, ICC, IF, IP
- Related Disease
- Squalene Synthase Deficiency; Smith-Lemli-Opitz Syndrome
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
Target
- Alternative Names
- SS; SQS; DGPT; ERG9; SQSD
- Gene ID
- 2222
- UniProt ID
- P37268
- Long Name
- Farnesyl-diphosphate farnesyltransferase 1
- Cellular Localization
- Endoplasmic reticulum membrane
- Post Translation Modifications
- Ubiquitination at Lys5, Lys117, Lys93, Lys138, Lys30, Lys41, Lys45, Lys170, Lys213, Lys318, and Lys358 (NX_P37268 [NX_P37268-1])
Modification sites at PhosphoSitePlus (P37268)
Glycosylation from GlyGen (P37268) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001274671.1; NP_001274672.1
- Function
- Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene.
Proceeds in two distinct steps.
In the first half-reaction, two molecules of FPP react to form the stable presqualene diphosphate intermediate (PSQPP), with concomitant release of a proton and a molecule of inorganic diphosphate.
In the second half-reaction, PSQPP undergoes heterolysis, isomerization, and reduction with NADPH or NADH to form squalene.
It is the first committed enzyme of the sterol biosynthesis pathway.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "FDFT1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1278 | Hi-Affi™ Rabbit Anti-FDFT1 Recombinant Antibody (clone DS1278AB) | ICC, IF, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0002U | Rabbit Anti-FDFT1 Recombinant Antibody (clone 10H12) | ELISA, WB, IHC | Rabbit IgG |
| VS3-FY542 | Recombinant Rabbit Anti-FDFT1 Antibody (clone R08-6B1) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY202 | Mouse Anti-NHP FDFT1 Recombinant Antibody (clone OTI2F10) | WB, IHC, IF | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2494 | Anti-FDFT1 Immunohistochemistry Kit | IHC |
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