Rabbit Anti-FOXA1 Recombinant Antibody (clone 3B2) (CAT#: ZG-0674J)
This product is a rabbit antibody that recognizes FOXA1.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-FOXA1 Antibody (ZG-0674J) in WB
Western Blot
Positive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate
All lanes: FOXA1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 50, 46 kDa
Observed band size: 50 kDa
IHC
Figure 2 Rabbit Anti-FOXA1 Antibody (ZG-0674J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC
Figure 3 Rabbit Anti-FOXA1 Antibody (ZG-0674J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IP
Figure 4 Rabbit Anti-FOXA1 Antibody (ZG-0674J) in IP
Immunoprecipitating FOXA1 in Hela whole cell lysate
Lane 1: Rabbit control IgG in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0674J (2µg)+ Hela whole cell lysate(500µg)
Lane 3: Hela whole cell lysate (10µg)
Specifications
- Immunogen
- A synthesized peptide derived from human FOXA1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human FOXA1
- Species Reactivity
- Human
- Clone
- 3B2
- Applications
- ELISA, WB, IHC, IP
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunoprecipitation.
WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000
Target
- Alternative Names
- HNF3A; TCF3A; hepatocyte nuclear factor 3-alpha; HNF-3-alpha; HNF-3A; TCF-3A; forkhead box protein A1; transcription factor 3A
- Gene ID
- 3169
- UniProt ID
- P55317
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4089z | Mouse Anti-FOXA1 Recombinant Antibody (clone 20D10) | WB, IP, IF, ELISA | Mouse IgG2a, κ |
| MOB-1057MZ | Recombinant Mouse Anti-Human FOXA1 Antibody | ICC/IF, WB | Mouse antibody |
| MRO-0599-CN | Rabbit Anti-FOXA1 Recombinant Antibody (clone JF10-02) | WB, IF, IHC | Rabbit IgG |
| VS13-YC414 | Rabbit Anti-FOXA1 Recombinant Antibody (VS13-YC414) | IHC-P | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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